The several industrial applications of lipases have stimulated interest in isolation of new enzymes from novel sources. In this study, the lipase production from Trichoderma atroviride 676 was optimized by statistical design methods. The response surface methodology based on 3(3-1) fractional factorial, showed that the yeast extract and MgSO4 played a significant role on lipase production, yiel...

A novel polyamine microsphere, prepared from the water-in-oil emulsion of polyethylenimine, was characterized. The investigation of scanning electron microscopy showed that the polyamine microsphere is a regular ball with a smooth surface. The diameter distribution of the microsphere is 0.37-4.29 μm. The isoelectric point of the microsphere is 10.6. The microsphere can adsorb proteins through t...

The lipase produced by a newly isolate Sporidiobolus pararoseus strain has potential catalysis ability for esterification reactions. In order to improve its synthetic activity, this work aimed at optimizing 'synthetic lipase' production by submerged fermentation of a conventional media based on peptone, yeast extract, NaCl and olive oil using experimental design technique. According to the resu...

Background: Lipase enzymes are of great importance in various industries. Currently, extensive efforts have been focused on exploring new lipase producer microorganism as well as genetic and protein engineering of available lipases to improve their functional features. Methods: For screening lipase-producing lactobacilli, isolated strains were inoculated onto tributyrin agar plates. Molecular ...

The binding of porcine pancreatic lipase and colipase was studied using the techniques of two-phase partition and affinity chromatography. The binding exhibited a pH dependency (maximum = pH 5.8, minimum = pH 8.6), was not significantly effected by the concentrations of NaCl, (0 to 1 M), and was inhibited by bile salts at concentrations above their critical micelle concentration. Below their cr...

In vitro, e-polylysine (EPL) strongly inhibited the hydrolysis of trioleoylglycerol emulsified with phosphatidylcholine (PC) and taurocholate by either pancreatic lipase or carboxylester lipase. The EPL concentration required for 50% inhibition of pancreatic lipase, 0.12 mM, was eight times lower than the concentration of orlistat required for the same effect. The 50% inhibition concentration b...

a new hybrid nanomaterial was developed by immobilization of phosphomolybdate anions on the surface of modified magnetite nanoparticles with quaternary phosphonium cations. silica coated magnetic nanoparticles supported phosphonium cations, ph3p+-scmnps, were prepared by covalent attachment of chloropropylsilyl groups on the surface of silica coated magnetite nanoparticles and next reaction wit...

Background: Magnetic separation using magnetic nanoparticles can be used as a simple method to isolate desulfurizing bacteria from a biphasic oil/water system. Objectives: Magnetite nanoparticles were applied to coat the surface of Rhodococcus erythropolis IGTS8 and Rhodococcus erythropolis FMF desulfurizing bacterial cells, and the viability and...