A method is described for the automated analysis of urinary 11-deoxy-17-oxo steroids from hydrolyzed conjugate extracts. Steroid conjugates are extracted manually on an Amberlite XAD-2 column and hydrolyzed with f3-glucuronidase at 62#{176}C for 90 mm. After addition of alkali, the samples are transferred to the AutoAnalyzer where the steroids are extracted into isooctane and estimated colorime...

A simplified procedure utilizing ferrous ammonium sulfate-thiourea as the reductant for the determination of inorganic phosphate in serum and urine by the AutoAnalyzer is described. Elimination of the heating bath usually required for many phosphorus methods for color development yields an improved S.D. of ± 0.25 and facilitates troubleshooting during any apparatus malfunction. Comparison of re...

NOMOGRAMS ARE used regularly in our laboratory for various routine calculations (1). A frequent calculation, which may be performed rapidly with the nomogram presented here, is typified by the colorimetric determination of glucose concentration with a reaction in which absorbance decreases as glucose concentration increases (Figure 1). The system uses an AutoAnalyzer and depends upon the reduct...

A new, precise, and accurate procedure is described for the automated determination of serum alkaline phosphatase on the Robot Chemist. The substrate, p-nitrophenylphosphate in 2-amino-2-methyl-1-propanol, offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. Other advantages are the small sample volumes required and linear reaction rates. C...

The continuous flow principle employed in the Technicon AutoAnalyzer has been adapted for the assay of anti-A and anti-B. The method has an acceptable degree of reproducibility and has been used, principally, for the quantitation of anti-A and anti-B in factor VIII concentrates of intermediate activity. It is, however, a method that can be applied to the assay of these antibodies in serum samples.

Total chromogen, true, and AutoAnalyzer methods of measuring serum and urine creatinine by the Jaffe reaction were investigated. Some factors influencing this reaction were examined. These included wavelength, blank, linearity, and conditions of color development. Modifications of the three methods were made and their precision, recovery, and sample stability determined. The interference of ket...

AIM Diabetic retinopathy (DR) is the most common preventable cause of blindness where early detection and treatment can be sight-saving. Search for biomarkers of the disease has been relentless. We aimed to determine whether lipoproteins apolipoproteins A1 and B1 (Apo-A1 and Apo-B1) have stronger associations with DR in contrast to conventionally measured low-density lipoprotein (LDL) and high-...

The direct procedure for determining serum cholesterol described by Wybenga et al. [C/in. Chem. 16, 980 (1970)] can be used to assay 29 samples in 10-mm reaction time by using the “CentrifiChem” parallel fast analyzer (Union Carbide). Commercial reference serum was used for standardization. Correlation of results with those obtained by the method of Abell et al. [J. Bio/. Chem. 195, 357 (1952)]...

We propose an automated method for the routine analysis of urinary iodide, using paired-ion reversed-phase HPLC with electrochemical detection and a silver working electrode. Assay conditions include a flow rate of 1.0 mL/min and an operating potential of 0.10 V. The retention time for iodide is 5.4 min. Sample preparation can be semiautomated by use of a reduced-pressure manifold. The detectio...

An eight-channel programmed flow system for automatic lipid extraction and TLC application is described. Each channel has a container for lipid extraction connected by Acidflex tubing through an AutoAnalyzer pump to a TLC applicator needle. Extraction containers are prepared from disposable Oxford sampler pipet tips by inserting a small cotton filter into their lower, narrower end, which is con...