Low molecular weight RNA species are described in isolated nuclear components and cytoplasm of salivary gland cells of Chironomus tentans. In addition to 4S and 5S RNA and RNA in the 4-5S range previously described, at least three other components in the range below 16S are present. RNA, the molecular weight of which was estimated to 2.3 x 10(5) and designed 10S RNA, can be observed only in nuc...

We report an on-line coupling of gel permeation chromatography (GPC) to Fourier transform mass spectrometry (FTMS) using a modified commercial electrospray ionization (ESI) interface. Selected oligomer profiles for the sodiated (1+ through 5+ charge states) oligomer ions of a narrow-molecular-weight poly(methyl methacrylate) were generated and used for obtaining a calibration curve. Using the M...

Concentrated solutions of low-molecular-weight DNA (Mw=35000) with a known molecular-weight distribution can be prepared in several hours, and require no additional fractionation procedures. This is achieved by sonication of the DNA in 1.0 M-NaCl at high power at 0--2 degrees C. No denaturation of the DNA is detectable, even after 8h of continuous sonication. After 2h, the molecular-weight dist...

Low molecular weight heparin-modified isoliquiritigenin-loaded solid lipid nanoparticle (LMWH-ISL-SLN) was developed for injective application. The morphological observation, particle diameter and zeta potential of LMWH-ISL-SLN were characterized using transmission electron microscopy (TEM) and a Malvern Zetasizer. Its entrapment efficiency (EE) and drug loading (DL) were determined by ultracen...

Due to unique applications of palladium nanoparticles, synthesis of these nanoparticles by a simple and low cost method is very important. In this work, Pd nanoparticles were synthesized with narrow size distribution by loading metal salt (Pd(OAc)2 ) into the polymeric matrix (PEG) as reducing agent and stabilizer. Also, the effect of metal salt concentration and PEG molecular weight on the con...

INTRODUCTION Dermatan sulfate (DS) is well-known for its anticoagulant activity through binding to heparin cofactor II to enhance antithrombin action. It has also been suggested that DS has a profibrinolytic effect, although the exact molecular mechanism is as yet unknown. MATERIALS AND METHODS An in vitro amidolytic method was used to study the effect of high and low molecular weight-DS on t...

The shape of a probability distribution is often summarized by the distribution’s skewness and kurtosis. Starting from a symmetric ”parent” density f on the real line, we can modify its shape (i.e. introduce skewness and in-/decrease kurtosis) if f is appropriately weighted. In particular, every density w on the interval (0, 1) is a specific weighting function. Within this work, we follow up a ...

The chromium/vanadium bimetallic Phillips catalysts developed by our research group have been proved to be a promising process to produce bimodal polyethylene using a single-reactor process. The vanadium loading of CrV-1/1, CrV-1/2, and CrV-1/3 has a significant effect on the polymerization activity, product molecular weight (MW), as well as th...