BACKGROUND A reliable quenching and metabolite extraction method has been developed for Lactobacillus plantarum. The energy charge value was used as a critical indicator for fixation of metabolism. RESULTS Four different aqueous quenching solutions, all containing 60% of methanol, were compared for their efficiency. Only the solutions containing either 70 mM HEPES or 0.85% (w/v) ammonium carb...

Ammonium acetate and ammonium chloride are the white crystalline solid inorganic compounds having wide application in synthesis and analytical chemistry. The aim of present study was to evaluate the impact of biofield treatment on spectral properties of inorganic salt like ammonium acetate and ammonium chloride. The study was performed in two groups of each compound i.e., control and treatment....

In this study, multi component Hantzsch reaction was carried out between various  Aromatic Aldehyde with Dimedone,Ethyl acetateand ammonium acetate to produce polyhydroquinolinederivatives under solvent-free conditions at 110oC by a (Zinc oxide  nanocatalyst) as effective Lewis acid. The remarkable advantages offered by this method compared with other methods are a green catalyst, si...

An aqueous solution of boric acid, as an efficient and green catalytic system, was efficiently utilized four-component cascade reaction of aryl aldehydes, dimedone, β-ketoesters and ammonium acetate to give hexahydroquinolines. By the reaction of boric acid with water, H+ was generated and efficiently catalyzed the reaction under mild and green condition. Also, optimization of the reaction cond...

An analytical method was developed for the accurate determination of benzoic acid in commercial quasi-drug drinks using isotope dilution liquid chromatography mass spectrometry (ID-LC/MS). Sample drinks were spiked with C6-benzoic acid and these mixtures were cleaned by a nylon membrane filter. The sample mixtures were then analyzed using LC/Tandem MS in the negative ion mode without further cl...

Objective: To develop and validate a sensitive, selective and reproducible method for the estimation of Itraconazole (ITR) and its pharmacologically active metabolite Hydroxy Itraconazole (HITR) in human CPDA (Citrate Dextrose Phosphate Adenine) plasma, using Loratadine (L) as an internal standard (IS). Methods: The drugs of interest and IS were extracted by solid phase extraction technique, us...

A simple, rapid, specific, sensitive and liquid chromatography coupled with tandem mass spectrophotometric method was developed and validated for the estimation of azathioprine and its metabolite 6-mercaptopurine in human plasma by using lamivudine and 6-mercaptopurine D3 as the internal standard. Azathioprine and 6mercaptopurine were extracted from human plasma by solid-phase extraction (SPE)-...

an ultrasound-assisted surfactant-enhanced emulsification microextra- ction (uaseme) coupled with high performance liquid chromatography-diode array detection has been developed for the extraction and determination of thiopental in serum and urine samples. a simple microextraction method based on emulsification of organic extraction solvent in aqueous sample using an ionic surfactant and withou...

A rapid and sensitive LC-MS/MS method based on the Triple Quad system has been developed and validated for the determination and pharmacokinetics of taxifolin and its nanodispersion in rat plasma. Taxifolin plasma samples along with butylparaben (internal standard) were pre-treated by liquid-liquid extraction with ethyl acetate, and then separated on a SB-C18 RRHD column (150 mm × 2.1 mm × 1.8 ...

Moisture content (MC) is one of the most important factors that can affect many physical and mechanical properties of wood and veneers. MC strongly affects the final strength and durability of joints, development of surface checks in the wood and dimensional stability of the bonded assembly. In this study, plywood panels made from Alnus glutinosa (Alder), Fagus orientalis (Beech) and Picea orie...