Serodiagnosis of cytomegalovirus (CMV) infection by complement fixation tests depends on showing a fourfold rise in antibody titer from acute- to convalescent-phase sera. Freeze-thaw and glycine-extracted, infected cell culture antigens used for these tests give markedly different titers in reactions with the same sera. In this study, we characterized the CMV-infected cell polypeptides containe...

[1] Permafrost soils store nearly half of global soil carbon (C), and therefore permafrost thawing could lead to large amounts of greenhouse gas emissions via decomposition of soil organic matter. When ice-rich permafrost thaws, it creates a localized surface subsidence called thermokarst terrain, which changes the soil microenvironment. We used soil profile CO2 measurements to understand the r...

The thermodynamic behavior of asphalt mixtures is critical to the engineers since it directly relates to the damage in asphalt mixtures. However, most of the current research of the freeze-thaw damage of asphalt mixtures is focused on the bulk body from the macroscale and lacks a fundamental understanding of the thermodynamic behaviors of asphalt mixtures from the microscale perspective. In thi...

A freeze-thaw cycle to -12 degrees C induced several physical and compositional changes in the microsomal membranes isolated from crown tissue of winter wheat (Triticum aestivum L. cv Frederick). Exposing 7-day-old, nonacclimated seedlings to a single freeze-thaw cycle prevented regrowth of the crown and resulted in increased membrane semipermeability. The phospholipid and protein content of mi...

Much of the world's boreal forest occurs on permafrost (perennially cryotic ground). As such, changes in permafrost conditions have implications for forest function and, within the zone of discontinuous permafrost (30-80% permafrost in areal extent), distribution. Here, forested peat plateaus underlain by permafrost are elevated above the surrounding permafrost-free wetlands; as permafrost thaw...

Seasonal frozen loess tunnel stability is controlled by many factors. To study the effects of water content and freeze-thaw cycles of loess tunnel stability, taking loess in Shanxi Yangqu No. 1 tunnel face as the research object, to do different moisture stability studies on loess tunnel after repeated freeze-thaw cycles. The results show that: (1) by the comprehensive structure potential and s...

Nitrate (NO3 (-) ) export coupled with high inorganic nitrogen (N) concentrations in Alaskan streams suggests that N cycles of permafrost-influenced ecosystems are more open than expected for N-limited ecosystems. We tested the hypothesis that soil thaw depth governs inorganic N retention and removal in soils due to vertical patterns in the dominant N transformation pathways. Using an in situ, ...

[1] Arctic warming is expected to increase thermokarst erosion in thaw lakes, thus inducing large emissions of CH4 to the atmosphere. To reduce uncertainties about the mechanisms, magnitude and timing of methane emissions, we conducted an in-situ experiment to simulate lake expansion following thermokarst erosion. Three tundra horizons were excavated, incubated at the bottom of a thaw lake and ...

The terrestrial cryosphere comprises cold areas of Earth’s land surface where water is either permanently or seasonally frozen. This includes most regions north of 40 degrees North latitude and most mountainous regions where elevation is greater than 1000m [1]. Permafrost is ground that perennially at or below 0 throughout the year. Above the permafrost is the active layer, which experiences su...

OBJECTIVES The stability of circulating proteins can be affected by repeated freezing and thawing. The aim of our study was to identify the effect of repeated freezing and thawing on the plasma and serum concentrations of eight proteins [interferon-γ, interleukin (IL)-8, IL-15, IL-17A, matrix metalloproteinase (MMP)-7, tumor necrosis factor-α, vascular endothelial growth factor (VEGF), and VEGF...