Transcription factors (TFs) act withinwider regulatory networks to control cell identity and fate. Numerous TFs, including Scl (Tal1) and PU.1 (Spi1), are known regulators of developmental and adult haematopoiesis, but how they act within wider TF networks is still poorly understood. Transcription activator-like effectors (TALEs) are a novelclassofgenetic toolbasedon themodularDNA-bindingdomain...

Transcription activator-like effectors (TALEs) contain modular programmable DNA binding domains. Fusing TALEs with effector domains creates synthetic transcription factors (TALE-TFs) or nucleases (TALENs), enabling precise gene manipulations. The construction of TALEs remains challenging due to their repetitive sequences. Here we report a simple TALE assembly reaction (STAR) that enables indivi...

Edgar Allan Poe’s tales offer a variety of instances linked to the analysis of human nature and the processes it goes through during the stories. Most of them treat about the destruction of the self as the narrator finds himself confronted with the darkness that gradually will come to annihilate reason and any sensible thinking. The protagonist witnesses not only the darkness inside, but also t...

Transcription activator-like effectors (TALEs) are a class of sequence-specific DNA-binding proteins that utilize a simple and predictable modality to recognize target DNA. This unique characteristic allows for the rapid assembly of artificial TALEs, with high DNA binding specificity, to any target DNA sequences for the creation of customizable sequence-specific nucleases used in genome enginee...

Transcription activator like effectors (TALEs) are injected via the type III secretion pathway of many plant pathogenic Xanthomonas spp. into plant cells where they contribute to disease or trigger resistance by binding to DNA and turning on TALE-specific host genes. Advances in our understanding of TALEs and their targets have yielded new models for pathogen recognition and defense. Similarly,...

TALE (transcription activator-like effector) proteins can be tailored to bind to any DNA sequence of choice and thus are of immense utility for genome editing and the specific delivery of transcription activators. However, to perform these functions, they need to occupy their sites in chromatin. In the present study, we have systematically assessed TALE binding to chromatin substrates and find ...

In the vertebrate central nervous system (CNS), specialized glial and neuronal cells positioned at the dorsal and ventral midline act as intermediate targets for commissural axons by secreting a variety of attractants and repellents. Despite the diversity of commissural projections, recent findings suggest that the same basic set of molecules controls midline crossing at all level of the CNS. M...

Today we have access to unprecedented amounts of literary texts. However, search still relies heavily on key words. In this paper, we show how sentiment analysis can be used in tandem with effective visualizations to quantify and track emotions in both individual books and across very large collections. We introduce the concept of emotion word density, and using the Brothers Grimm fairy tales a...

The ability to conditionally rewire pathways in human cells holds great therapeutic potential. Transcription activator-like effectors (TALEs) are a class of naturally occurring specific DNA binding proteins that can be used to introduce targeted genome modifications or control gene expression. Here we present TALE hybrids engineered to respond to endogenous signals and capable of controlling tr...