A moderately thermophilic, facultatively anaerobic, amylolytic bacterium was isolated from palm wine, a tropical alcoholic beverage that was sampled in Senegal. The cells were gram positive, catalase positive, non-spore forming, rod shaped, and slightly motile with peritrichous flagella. The strain which we examined did not possess cytochrome and produced L-(+)-lactate, acetate, ethanol, and fo...

BACKGROUND The gram-positive bacterium Bacillus methanolicus MGA3 is a promising candidate for methanol-based biotechnologies. Accurate determination of intracellular metabolites is crucial for engineering this bacteria into an efficient microbial cell factory. Due to the diversity of chemical and cell properties, an experimental protocol validated on B. methanolicus is needed. Here a systemati...

F(o)F(1)-ATP synthase (F(o)F(1)) synthesizes ATP in the F(1) portion when protons flow through F(o) to rotate the shaft common to F(1) and F(o). Rotary synthesis in isolated F(1) alone has been shown by applying external torque to F(1) of thermophilic origin. Proton-driven ATP synthesis by thermophilic Bacillus PS3 F(o)F(1) (TF(o)F(1)), however, has so far been poor in vitro, of the order of 1 ...

A cellulolytic, thermophilic actinomycete (previously isolated from municipal refuse compost samples) was identified as Thermomonospora curvata. A determination was made of the optimal conditions for cellulase production by T. curvata when grown at 55 C in a medium containing mineral salts, cellulose, and yeast extract. The pH and temperature optima (pH 6.0 and 65 C) for the cellulase produced ...

The ability to hydroxylate steroids is not common amongst bacilli, but a thermophilic strain hydroxylates progesterone to form both the 6 a and 60 derivatives. The hydroxylation products formed at 55°C were extracted, purified by TLC and HPLC and identified by 'HNMR. The hydroxylation was inhibited by ketoconazole and gave a reduced CO spectrum peak at 449 nm and a progesterone binding spectrum...

A prototrophic, thermophilic bacillus is in a state of biotin insufficiency when grown in medium consisting of inorganic salts and a carbon source. The effect of this biotin deficiency on the growth rate is severe only if the functioning of pyruvate carboxylase is essential for the utilization of the particular growth substrate. A mutant, PC2, of the thermophile devoid of active pyruvate carbox...

The purpose of this study is to create an assortment whole grain wheat bread that resistant microorganisms and has high nutritional value. research was carried out on the microbiological purity flour, inhibitory effect plant extracts thermophilic bacteria detected in flour amount biologically active additives vegetable raw materials product. revealed thermostable types flour. 1 g found contain ...

Campylobacter jejuni is a Gram negative, curved, motile, thermophilic and microaerophilic bacillus in Campylobacteriaceae family, which is one of the main causes of enteritis in human named campylobacteriosis. The main reservoir of Campylobacter jejuni is the alimentary tract of animals, especially chicken and turkey. Consumption of semi-cooked chicken meat, raw milk and unchlorinated water are...