The engineering of thermostable enzymes is receiving increased attention. The paper, detergent, and biofuel industries, in particular, seek to use environmentally friendly enzymes instead of toxic chlorine chemicals. Enzymes typically function at temperatures below 60°C and denature if exposed to higher temperatures. In contrast, a small portion of enzymes can withstand higher temperatures as a...

Geobacillus stearothermophilus SR74 is a locally isolated thermophilic bacteria producing thermostable and thermoactive α-amylase. Increased production and commercialization of thermostable α-amylase strongly warrant the need of a suitable expression system. In this study, the gene encoding the thermostable α-amylase in G. stearothermophilus SR74 was amplified, sequenced, and subcloned into P. ...

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2, the causative agent of coronavirus disease (COVID-19)) has caused relatively high mortality rates in humans throughout world since its first detection late December 2019, leading to most devastating pandemic current century. Consequently, SARS-CoV-2 therapeutic interventions have received priority from public health authorities. Despi...

snail is one of the worst causes of food allergy. thus, the aim of this study was to identify the major and minor allergens of the local marine snail ( cerithidea obtusa ) and subsequently to investigate the impacts of heat treatment on the ige-binding activity of snail allergens. proteins from raw and heat-treated snails (boiled, roasted and fried) were extracted and then resolved by sodium do...

polyphenol oxidase (ec. 1.10.3.1 ppo), an ionically unbound and thermostable enzyme, was extracted from the leaves of cinnamomum tamala having aroma flavor used to add in indian food and vegetables as a part of spices to enhance the taste of recipes. the enzyme was purified 2.63-fold with a total yield of 9.5% by ammonium sulfate precipitation followed by sephadex g-100 gel filtration chromatog...

In January 2003, the Commonwealth G o v e rnment commenced the National Meningococcal C Vaccination Program. This p rogram provides free meningococcal C conjugate vaccine over the next four years to all children and adolescents who turn one to 19 years of age in 2003, a target group of almost 6 million. The meningococcal C vaccine has also been added to the Australian Standard Vaccination Sched...

Two oil emuisified vaccines of Newcaslte disease were prepared from Lasota strain. The virus was propagated in 9-day-old chicken embryos through allantoic route. The haemagglutination titre of the allantoic fluid used for vaccine preparation was 2048. The fluid was inactivated by 0.1 per cent f nal concentration of formalin at 37 oc for 6 hours. Inactivated allantoic fluid was emulsified with a...