The adenosine A2A receptor (A2AR) belongs to the superfamily of membrane proteins called the G-protein-coupled receptors (GPCRs) that form one of the largest superfamilies of drug targets. Deriving thermostable mutants has been one of the strategies used for crystallization of A2AR in both the agonist and antagonist bound conformational states. The crystal structures do not reveal differences i...

BACKGROUND The concerted action of three complementary cellulases from Clostridium thermocellum, engineered to be stable at elevated temperatures, was examined on a cellulosic substrate and compared to that of the wild-type enzymes. Exoglucanase Cel48S and endoglucanase Cel8A, both key elements of the natural cellulosome from this bacterium, were engineered previously for increased thermostabil...

Halophilic archaeon Halolamina pelagica CDK2, showcasing plant growth-promoting properties and endurance towards harsh environmental conditions (high salinity, heavy metals, high temperature UV radiation) was sequenced earlier. Pan-genome of genus created investigated for strain-specific genes which might confer it with features helping to withstand abiotic stress. Pathways subsystems in CDK2 w...

Background: Pectinases are pectin degrading class of enzymes including polygalacturonase (PG), polymethyl galacturonase (PMG), pectate lyase (PEL), and pectin esterase (PE) that are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juices. Objectives: A highly methylated pectin degrading bacterium from soil covered with fruit wa...

background and objectives: thermophilic bacteria are less studied but important group of microorganisms due to their ability to produce industrial enzymes. materials and methods: in this study, thermophilic bacteria were isolated from hot spring of tarabalo, india. a bacterium that could tolerate high temperatures was characterized by morphology, biochemistry and sequencing of its 16s rrna gene...

methicillin-resistant staphylococcus aureus (mrsa) is the main cause of hospital infection emerged over the last decades. rapid detection of mrsa is important for patient care and proper usage of infection control. detection of meca genes (encoding resistance to methicillin and other similar antibiotics) and nuc genes (encoding staphylococcal thermostable nuclease) by pcr method is now consider...