Acyl shifts involving N-S and S-S rearrangements are reactions central to the breaking of a peptide bond and forming of thioester intermediates in an intein-catalyzed protein splicing that ultimately leads to the formation of a new peptide bond by an uncatalyzed S-N acyl shift reaction. To mimic these three acyl shift reactions in forming thioesters and the subsequent peptide ligation, here we ...

In the beta-proteobacterium Azoarcus evansii, the aerobic metabolism of 2-aminobenzoate (anthranilate), phenylacetate, and benzoate proceeds via three unprecedented pathways. The pathways have in common that all three substrates are initially activated to coenzyme A (CoA) thioesters and further processed in this form. The two initial steps of 2-aminobenzoate metabolism are catalyzed by a 2-amin...

Thiamine (vitamin B1) is essential for brain function because of the coenzyme role thiamine diphosphate (ThDP) in glucose and energy metabolism. In order to compensate deficiency, several precursors with higher bioavailability were developed since 1950s. Among these, thioester benfotiamine (BFT) has been extensively studied beneficial effects both rodent models neurodegeneration human clinical ...