Ultraviolet (UV) radiation is a major inducer of skin aging and accumulated exposure to UV radiation increases DNA damage in skin cells, including dermal fi broblasts. In the present study, we developed a novel DNA repair regulating material discovery (DREAM) system for the high-throughput screening and identifi cation of putative materials regulating DNA repair in skin cells. First, we establi...

An important task in many scientific and engineering disciplines is to set up experiments with the goal of finding the best instances (substances, compositions, designs) as evaluated on an unknown target function using limited resources. We study this problem using machine learning principles, and introduce the novel task of active k-optimization. The problem consists of approximating the k bes...

An early step in the drug discovery involves screening through numerous chemical compounds to find molecules that have a specified biological or biochemical effect. This screening process is costly, thus any modifications to make the screen more efficient would reduce the total cost of drug discovery. Current high-throughput screening methods test each compound from a library of compounds indiv...

Biology has always been a heavily technology limited field. Burgeoning fields such as systems biology require the development and implementation of new technologies, enabling high-throughput and high-fidelity measurements of large systems. Microfluidics promises to fulfil many of the requirements put forth. Here I will discuss the various approaches employed to date for performing high-throughp...

BACKGROUND Radiotherapy is one of the mainstays in the treatment for cancer, but its success can be limited due to inherent or acquired resistance. Mechanisms underlying radioresistance in various cancers are poorly understood and available radiosensitizers have shown only modest clinical benefit. There is thus a need to identify new targets and drugs for more effective sensitization of cancer ...

Yeast two-hybrid (Y2H) screening methods are an effective means for the detection of protein-protein interactions. Optimisation and automation has increased the throughput of the method to an extent that allows the systematic mapping of protein-protein interactions on a proteome-wide scale. Since two-hybrid screens fail to detect a great number of interactions, parallel high-throughput approach...

High-throughput screening is a common strategy used to identify compounds that modulate biochemical activities, but many approaches depend on cumbersome fluorescent reporters or antibodies and often produce false-positive hits. The development of "label-free" assays addresses many of these limitations, but current approaches still lack the throughput needed for applications in drug discovery. T...

Fig. 1: Steps during process development. HTPD as a first step can help to generate a large knowledge space and improve process understanding. That advances the quality-by-design concept regarding the FDA’s (Food and Drug Administration) initiative “PharmaceuticalcGMPs for the 21 century” and ICH International Conference on Harmonisation of technical requirements for the registration of pharmac...

The aims of high-throughput (HTP) protein production systems are to obtain well-expressed and highly soluble proteins, which are preferred candidates for use in structure-function studies. Here, we describe the development of an efficient and inexpensive method for parallel cloning, induction, and cell lysis to produce multiple fusion proteins in Escherichia coli using a 96-well format. Molecul...

Ion channels present a group of targets for major clinical indications, which have been difficult to address due to the lack of suitable rapid but biologically significant methodologies. To address the need for increased throughput in primary screening, the authors have set up a Beckman/Sagian core system to fully automate functional fluorescence-based assays that measure ion channel function. ...