OBJECTIVE In order to complete the knowledge of the genomic organization of the human thyroglobulin gene, the present work was designed to establish the intron-exon organization from exon 24 to exon 35 and to construct a more complete physical map of the gene. DESIGN Screening of two genomic libraries, and subsequent restriction mapping, hybridization and sequencing were used to characterize ...

OBJECTIVE The aim of the present study was to examine whether interactions between polymorphisms in the thyroglobulin and ADAM metallopeptidase with thrombospondin type 1 motif, 16 (ADAMTS16) genes are associated with the development of premature ovarian failure (POF). METHODS A total of 75 patients with POF and 196 controls were involved in this study. We used a GoldenGate assay to genotype ...

When rabbits were injected with 10.0 mg rabbit thyroglobulin in complete Freund's adjuvant, the earliest thyroid lesions were seen on day 5 and uniformly severe thyroid lesions were seen by day 14; these observations were not significantly different from the thyroid lesions observed at 1 and 2 months post-immunization. Pooled sera were obtained from immunized, thyroidectomized, and nonthyroidec...

The dissociation of thyroid 27 S iodoprotein by sodium dodecyl sulfate (SDS) and by succinic anhydride was investigated by means of ultracentrifugation and polyacrylamide gel electrophoresis. The iodoprotein obtained from either a human or hog was dissociated into three kinds of subunits (S-19, S-17 and S-12) by SDS treatment. At increased concentrations of SDS, the S-12 subunit was predominant...

It has been assumed that denaturation occurs preliminary to enzymatic hydrolytic fission in some proteins. The basis of this supposition is the generally accepted observation that these proteins arc less readily attacked by proteolytic enzymes in their native state than when they are denatured (l-3). In the case of such readily digestible proteins as gelatin, prolamin, or casein, Linderstrom-La...