Adult stem/progenitor cells are found in different human tissues. An in vitro cell culture is needed for their isolation or for their expansion when they are not available in a sufficient quantity to regenerate damaged organs and tissues. The level of complexity of these new technologies requires adequate facilities, qualified personnel with experience in cell culture techniques, assessment of ...

Background: With all the recent advances in cancer treatments, many young cancer patients find themselves facing the prospect of losing their fertility after aggressive chemotherapy or radiotherapy. Cryopreservation of ovarian cortical tissue has emerged as a potential option to restore fertility in these young women. Materials and Methods: Because autotransplantation of cryopreserved ovarian c...

Rotating bioreactors such as the High Aspect Ratio Vessel (HARV) provide a low shear and gentle mixing environment, ideal for mammalian cell culture in 3D. The HARV is a cylindrical “disc-shaped” batch culture vessel with no internal moving parts, that rotates about a single axis. Oxygenation is provided by a permeable silicon rubber membrane, allowing the diffusion of gases to and from the med...

background and objectives: the aim of this study was to compare direct microscopic examination with culture and pcr for the diagnosis of mucorales infection in blood and tissue specimens. material and methods: blood samples and tissue specimens were obtained from 28 patients (total 58 samples) with suspected invasive fungal infection and cultured on proper media. direct smear of tissue samples ...

1. Human placental cord serum contains a factor or factors necessary for active growth of fibroblasts in tissue culture and a factor which prevents the appearance of a characteristic toxicity. 2. The growth-promoting factor(s) is stable to heating to 60 degrees C. for 30 minutes and is associated with the euglobulin fraction of the serum. 3. Extraction of dried cord serum with benzene, chlorofo...

A suspension of Ketawaspreparedby trypsinization of stock cultures in Blake bottles. After determining the cell count with a hemocytometer the suspension was diluted with medium to give a c@int of' 75,000 cells per 0. 9 ml. Using an automatic pi pette, 0. 9 m:1@. of the Hela suspension was delivered into each of the cups of the plastic panel and the test compound added in 0.1 ml. saline or in 0...

Mycoplasmas are frequently found as contaminants in tissue-cultured cells. Infections may be inapparent or cause severe cytopathic changes. The source of primary contaminations in most cases is probably the upper respiratory tract of man. The wide dissemination of infections in cultures most probably occurs as a result of aerosols set up during the processing of contaminated cultures. Mycoplasm...

The hanging drop tissue culture is a technique utilized in embryology [4] and other fields to allow growth that would otherwise be restricted by the flat plane of culture dishes and also to minimize the surface area to volume ratio, slowing evaporation. The classic hanging drop culture is a small drop of liquid, such as plasma or some other media allowing tissue growth, suspended from an invert...

A list, with titles, of about 120 papers by the author and his colleagues is followed by a classified list of important contributions by others in the fields discussed. Author and subject indices are given. Adventures in Respiration is not a physiological monograph in the usual sense, but rather the scientific autobiography of one of America's leaders in the physiology of respiration. The layma...