a thermodynamic study on the interaction between zinc ion (zn2+) and human growth hormone(hgh) was studied at two temperatures of 27c and 37c in aqueous solution using an isothermal titrationcalorimetry. it was found that there is a set of three identical and non-interacting binding sites for zn2+ ions. the intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 1.54...

Proteins can be bound to surfaces through chemical coupling reactions between the reactive groups of the protein and matrix. In this report, ribonuclease A immobilized on Silica beads, has been used as a model to study the modification of the properties of the enzyme after immobilization. The biological activity has been monitored by studying the binding of the inhibitor ~ytidine-3~-monophospha...