Diffusion anisotropy was investigated in the developing rat brain [postnatal day (P)6-29] with the use of ion-selective microelectrodes to measure the three-dimensional distribution of tetramethylammonium (TMA+) iontophoresed into the extracellular space (ECS). The diffusion parameters, ECS volume fraction alpha (alpha = ECS volume/total tissue volume), tortuosity lambda (lambda2 = apparent dif...