BACKGROUND Considering previous result in Non-Small Cell Lung Cancer (NSCLC), we investigated in human cancer cells the role of PARP3 in the regulation of telomerase activity. METHODS We selected A549 (lung adenocarcinoma cell line) and Saos-2 (osteosarcoma cell line), with high and low telomerase activity levels, respectively. The first one was transfected using a plasmid construction contai...

Introduction This study aimed to investigate the preventive and therapeutic effects of crocetin (Cro) on ischaemic stroke in cell animal models. Material methods A model oxygen glucose deprivation (OGD) a rat middle cerebral artery occlusion were established simulate stroke. The infarct volume was measured by TTC assay, apoptotic number counted TUNEL. Relative protein gene expression levels rat...

Development of an estrogen receptor-mediated, chemical-activated luciferase reporter gene-expression (ER-CALUX) assay was attempted by stable transfection of luciferase reporter genes in a number of cell lines. Stable transfection of the chimeric Gal4 estrogen receptor and luciferase gene constructs in MCF-7 breast cancer and Hepa.1c1c7 mouse hepatoma cell lines, as well as transfection of a ne...

We investigated the impact of nuclear factor erythroid 2–related 2 (Nrf2) overexpression in renal proximal tubular cells (RPTCs) on blood glucose, kidney injury, and sodium–glucose cotransporter (Sglt2) expression diabetic Akita Nrf2−/−/Nrf2RPTC transgenic (Tg) mice. Immortalized human RPTCs (HK2) stably transfected with plasmid containing SGLT2 promoter kidneys from patients diabetes were also...

Purpose: To investigate the effect of forkhead box N3 (FOXN3) protein on doxorubicin (DOX) resistance urothelial carcinoma (BLCA).
 Methods: Bioinformatics prediction and immunoblotting were used to evaluate FOXN3 expression in BLCA tissues cells. The overexpression was achieved by cell transfection. effects DOX apoptosis determined immunoblotting, assay, flow cytometry, while applied SIRT...

Alteration of adipocyte function contributes to the pathogenesis metabolic diseases including Type 2 diabetes and insulin resistance. This highlights need better understand molecular mechanism involved in dysfunction develop new therapies against obesity-related diseases. Modulating expression proteins micro-RNAs adipocytes remains highly challenging. paper describes a protocol differentiate mu...

The aim of the present study was to determine the effect of downregulating the expression of glucose-regulated protein 78 (Grp78) and Grp94 upon the rate of proliferation and apoptosis in the human gastric cancer SGC-7901 cell line. The SGC-7901 cells were divided into three groups as follows: i) An experimental group co-transfected with the small interfering RNA vectors, psiSTRIKE™/Grp78 and p...

Objective(s): Although low-dose radiotherapy (RT) that involves low collateral damage is more suitable for hepatocellular carcinoma (HCC) than traditional high-dose RT, but to achieve satisfactory therapeutic effect with low-dose RT, it is necessary to sensitize HCC cells to irradiation. This study was aimed to determine whether radiosensitivity of HCC cells can be enhanced using miR-26b by tar...

Background: IFN-g is mostly secreted by activated CD4+ , CD8+ T cells and NK cells. This cytokine has immunomodulatory, anti-cancer and anti-microbial effects and is important for prophylaxis, diagnosis and treatment of chronic infections and cancers. Objective: The purpose of this study was to clone the full cDNA of human IFN-g and express it in CHO cell line. Methods: Lymphocytes from a healt...