1. Chloroplast Inheritance 1.1. Early Cytological Observations of Chloroplast Inheritance 1.2. Chloroplast Inheritance in Isogamous Organisms 1.2.1. Unicellular Green Algae: Chlamydomonas reinhardtii 1.2.2. Discovery of Chloroplast DNA 1.2.3. Molecular Mechanism of Non-Mendelian Inheritance of cpDNA 1.2.4. Transformation of cpDNA 1.2.5. Active Digestion of mt– cpDNA Revealed in a Single Zygote ...

During development, positional values have to be assigned to groups of cells. The murine Hox genes are a class of genes that are predicted to be involved at some stage in this process. During embryogenesis they are expressed in distinct overlapping region- and stage-specific patterns and therefore must be regulated in response to positional information. In this study, we have analysed the activ...

High activity levels of a transgene can be very useful, making a transgene easier to evaluate for safety and efficacy. High activity levels can also increase the economic benefit of the production of high value proteins in transgenic plants. The goal of this research is to determine if recurrent selection for activity of a transgene will result in higher activity, and if selection for activity ...

Single inheritance has been widely accepted in the current programming practice to avoid the complication that incurred by multiple inheritance. Single inheritance enhances the reusability of codes and eliminates the confusion of identical methods that possibly defined in two superclasses. However, the mechanism of inner class in Java potentially reintroduces the problems encountered by multipl...

The gastrin gene is expressed in fetal pancreatic islet cells, but in the adult is expressed mainly in the gastric antrum. To study the regulation of the gastrin promoter, we created several transgenes containing the human and rat gastrin 5' flanking regions joined to the coding sequences of the human gastrin gene. The human gastrin transgene contained 1,300 bp of 5' flanking DNA, while the rat...

The molecular mechanisms that establish and maintain transcriptional differences between cardiomyocytes in the left and right ventricular chambers are unkown. We have previously analysed a myosin light chain 3f transgene containing an nlacZ reporter gene, which is transcribed in left but not right ventricular cardiomyocytes. In this report we examine the mechanisms involved in maintaining regio...

Hemophilia is a monogenic mutational disease affecting coagulation factor VIII or IX genes. The palliative treatment of choice based on the use safe and effective recombinant clotting factors. Advanced therapies will be curative, ensuring stable durable concentrations defective circulating factor. Results have so far been encouraging in terms levels times expression using mainly adeno-associate...