The objective of this study was to examine the post-thaw effects of three cryoprotective extenders (Tris-fructose-citric acid extender, Tris-glucose-citric acid extender, and lactose extender), three linear freezing rates (-1, -6, and -20 degrees C/min), and three thawing rates (37 degrees C water bath for 120s, 60 degrees C water bath for 30s, and 70 degrees C water bath for 8s) on coyote sper...

A concentrated sulfuric acid dissolution technique and a GC method are described for the estimation of tributyl phosphate, tris(2-chloroethyl) phosphate, tris(chloropropyl) phosphate, tris(1,3-dichloro-2-propyl) phosphate, triphenyl phosphate and tris(butoxyethyl) phosphate in soft polyurethane foam. A soft polyurethane foam sample containing organophosphoric acid triesters was dissolved in con...

Iron is an essential nutrient for many microbes. According to the "Trojan Horse Hypothesis", biological systems have difficulties distinguishing between Fe(3+) and Ga(3+), which constitutes the antimicrobial efficacy of the gallium(iii) ion. Nine novel tris(quinolono)gallium(iii) complexes and their corresponding iron(iii) analogs have been synthesized and fully characterized. Quinolone antimic...

Using restriction endonucleases which preferentially digest mouse main band DNA and leave satellite DNA intact, we have isolated highly purified chromatin fractions containing only mouse satellite or main band DNA. Following the digestion of mouse brain nuclei with EndoR Alu I, main band DNA chromatin is selectively extracted with 10mM Tris, 10mM EDTA. Satellite DNA chromatin is subsequently ex...

Oligoribonucleotides have been synthesized directly from unprotected ribonucleosides by a chemical polymerization approach using phosphorus tris-azoles. The procedure involves two steps: (i) the reaction of unprotected ribonucleoside with phosphorus tris-azole and (ii) the in situ oxidation of the resulting phosphite with iodine and water. Several phosphorus tris-azoles were investigated for ge...

Synthesis and spectral evaluation of new zinc and copper unsymmetrical mesoporphyrinic complexes are reported. Zn(II)-5-(4-acetoxy-3-methoxyphenyl)-10,15,20- tris-(4-carboxymethylphenyl)porphyrin, Zn(II)-5-[(3,4-methylenedioxy)phenyl]-10,15,20- tris-(4-carboxymethylphenyl)porphyrin, Cu(II)-5-(4-acetoxy-3-methoxyphenyl)-10,15,20- tris-(4-carboxymethylphenyl)porphyrin and Cu(II)-5-[(3,4-methylene...

The decay of ONOOH near neutral pH has been examined as a function of isomerization to H(+) and NO3(-), and decomposition to NO2(-) and O2via O2NOO(-). We find that in phosphate buffer k(isomerization) = 1.11 ± 0.01 s(-1) and k(disproportionation) = (1.3 ± 0.1) × 10(3) M(-1) s(-1) at 25 °C and I = 0.2 M. In the presence of 0.1 M tris(hydroxymethyl)aminomethane (Tris), the decay proceeds more ra...

The aim of this study was to determine the effects of the antioxidants superoxide dismutase (SOD),glutathione peroxidase (GPx) and butylated hydroxytoluene (BHT) on microscopic semen parameters andlipid peroxidation following the freeze-thawing of bull semen. Ejaculates were collected from five Holsteinbulls and pooled at 37°C. The semen samples were diluted with a CEY extender containing addit...

the aim of this study was to determine the effects of the antioxidants superoxide dismutase (sod),glutathione peroxidase (gpx) and butylated hydroxytoluene (bht) on microscopic semen parameters andlipid peroxidation following the freeze-thawing of bull semen. ejaculates were collected from five holsteinbulls and pooled at 37°c. the semen samples were diluted with a cey extender containing addit...

Circularly polarized luminescence (CPL) of unsubstituted helicenes is weak due to their low fluorescence quantum yield (Φf) and small dissymmetry factor (gCPL). In this study, we designed synthesized a donor-acceptor type [5]helicene, 5,10-dicyano-2,13-bis(3,4,5-tris(n-octyloxy)phenyl)[5]helicene. This helicene showed high (Φf = 0.63) good |gCPL| (4.2 × 10−3) symmetry-allowed transition its hel...