Rotenone is a widely used botanical insecticide, which can inhibit the mitochondrial respiratory chain of various insect cells, while mechanism its toxicity to cells needs further investigation. The purpose this study was understand level low (0.2 µg/mL) and high (20 concentrations rotenone in Spodoptera litura (SL-1) using trypan blue Coomassie brilliant staining assays. Our found that possess...

Background: Today, stem cells are the best candidates for cell therapy and tissue engineering. Adipose-derived Stem Cells (ADSCs) an essential source of in replacement therapies many diseases. Objectives: This study compared proliferation ADSCs alginate fibrin scaffolds. Methods: were isolated from adipose cultured or scaffolds with a medium containing PRP 10% FBS 10%. Then, viability percentag...

BACKGROUND Khat (Catha edulis) is a controversial plant having a euphoretic effect, at the same time part of culture in many countries such as Africa and Arabian Peninsula. The presence of amphetamine-like substance, cathinone and cathine make this plant banned in many countries. Many neurological and other system related studies have been carried out in this plant, but the lack of toxicity stu...

BACKGROUND In the present study, we describe a procedure to cryopreserve the postnatal members of the Leydig cell lineage, including progenitor (PLC), immature (ILC) and adult (ALC) Leydig cells from, respectively 21-, 35- and 90-day-old rats. METHODS The cells were resuspended in a culture medium supplemented with 1% bovine serum albumin (Dulbecco's Modified Eagle's Medium [DMEM]/F12) to a f...

BACKGROUND Cryptococcus neoformans is the commonest cause of fungal meningitis, with a substantial mortality despite appropriate therapy. Quantitative culture of cryptococci in cerebrospinal fluid (CSF) during antifungal therapy is of prognostic value and has therapeutic implications, but is slow and not practicable in many resource-poor countries. METHODS We piloted two rapid techniques for ...

Crude toxin was prepared by lyophilization and extraction of toxic Microcystis aeruginosa from four natural sources and a unicellular laboratory culture. The responses of cultures of liver (Mahlavu and PCL/PRF/5), lung (MRC-5), cervix (HeLa), ovary (CHO-K1), and kidney (BGM, MA-104, and Vero) cell lines to these preparations did not differ significantly from one another, indicating that toxicit...