Abstract Chagas disease is a zoonosis caused by Trypanosoma cruzi and transmitted triatomine bugs. Autochthonous to Latin America, has spread globally through travel migration. New drugs are needed urgently, in particular that cure the chronic stage. This where high-content imaging makes key contribution: assays with fluorescent parasites cell culture allow determine pharmacodynamic parameters ...

The hemoflagellate Trypanosoma cruzi is the causative agent of American trypanosomiasis. Despite the importance of motility in the parasite life cycle, little is known about T. cruzi motility, and there is no quantitative description of its flagellar beating. Using video microscopy and quantitative vectorial analysis of epimastigote trajectories, we find a forward parasite motility defined by t...

The fluid mechanics of the flow of blood through the labrum of Glossina is described in uninfected and trypanosome infected flies. The flow is characterised by the Reynolds number, and a frequency parameter for the pulsating flow and non-newtonian viscosity effects are considered. The effects of colonies of trypanosomes on the flow rate in the labrum and the interactions between colonies of Try...

OBJECTIVE Collection of triatomines in domestic, peridomestic and sylvatic environments in states of Bahia and Rio Grande do Sul, Northeastern and Southern Brazil respectively, and isolation of Trypanosoma cruzi strains. METHODS First, the captured triatomines were identified using insect identification keys, then their intestinal content was examined by abdominal compression, and the samples...

A series of 32 structurally diverse MGBs, derived from the natural product distamycin, was evaluated for activity against Trypanosoma brucei brucei. Four compounds have been found to possess significant activity, in the nanomolar range, and represent hits for further optimisation towards novel treatments for Human and Animal African Trypanosomiases. Moreover, SAR indicates that the head group l...

Trypanosomes must sense and respond to environmental change in order to progress through their life cycles. The American trypanosome, Trypanosoma cruzi, differentiates from the noninfective epimastigote form to the infective metacyclic form spontaneously in axenic culture. Here, we investigate the initial stimulus for that change and demonstrate that T. cruzi epimastigotes sense limitation of g...

The CL-14 clone was obtained from Trypanosoma cruzi CL strain by CHIARI and under certain culture conditions can present high metacyclogenesis rates, but its metacyclics have been considered noninfective for mice, as evaluated by parasitemia, hemoculture and xenodiagnosis, and also unable to determine tissue parasitism or histopathological lesions, despite heavy inocula used in attempts to infe...