Although alternative test methods based on the 3Rs (Replacement, Reduction, Refinement) are being developed to replace animal testing in reproductive and developmental toxicology, they are still in an early stage. Consequently, we aimed to develop alternative test methods in male animals using mouse spermatogonial stem cells (mSSCs). Here, we modified the OECD TG 489 and optimized the in vitro ...

BACKGROUND Epigallocatechin-3-gallate (EGCG) is a polyphenol constituent present in green tea previously shown to inhibit cancer growth. However, studies on human ovarian cancer are limited. This study evaluated, the effects of EGCG as a potential anti-proliferative and pro-apoptotic agent in the human ovarian cancer line, SKOV-3. MATERIALS AND METHODS The MTS assay which measures metabolic a...

BACKGROUND Both cadmium (Cd) and bisphenol A (BPA) are commonly encountered in humans' daily activities, but their combined genotoxic effects remain unclear. METHODS In the present study, we exposed a mouse embryonic fibroblast cell line (NIH3T3) to Cd for 24 h, followed by a 24 h BPA exposure to evaluate toxicity. The cytotoxicity was evaluated by viability with CCK-8 assay and lactate dehyd...

background: pectin is composed of complex polysaccharides that can inhibit cancer metastasis and proliferation with no evidence of toxicity. in the present study, the apoptotic and necrotic effects of pectic acid (pa) on the rat pituitary gh3/b6 tumor cells has been investigated. methods: gh3/b6 cells were cultured in the ham’s f12 medium enriched with 15% horse serum and 2.5% fetal bovine seru...

objective: although arsenite is toxic it is currently recommended for the treatment of malignancies. in this study the effects of sub-micromolar concentrations of sodium arsenite on the viability, morphology and mechanism of cell death of rat bone marrow mesenchymal stem cells (bmcs) over 21 days was investigated. materials and methods: in this experimental study, bmcs were extracted in dulbecc...

Pyridostigmine bromide (PB), a cholinesterase inhibitor used as a prophylactic against nerve agents, has been reported to produce neuronal apoptosis in rats. The goal of this study was to determine if PB produced similar levels of apoptosis in a C57BL/6J mouse model. Since the ability of PB to cross the blood-brain barrier is controversial, we used physostigmine (PHY), a cholinesterase inhibito...

The study investigated the underlying molecular mechanisms of ethanol extract of Annona muricata. L induced liver cancer cell apoptosis. The anti-proliferative effect of ethanol extract of Annona muricata. L was evaluated by MTT assay. Flow cytometry was used to detect the cell cycle and apoptosis. TUNEL assay was used to confirm cell apoptosis. DCFH-DA fluorescent probe can detect the level of...

the aim of this study was to examine the effect of crude bile on the human hepg2 and ccrf-cem cell lines. cells were exposed to different dilutions of bile. antiproliferative effects were determined by the cytotoxic mtt assay. cells undergoing apoptosis were identified by terminal deoxynucleotidyl transferase dutp nick end labeling (tunel) assay. bile administration resulted in dose-dependent c...

The long non-coding RNA (lncRNA) RUSC1-AS1 has been reported to be dysregulated in the progression of many cancers. Also, had detected highly expressed laryngeal squamous cell carcinoma and breast cancer cells, suggesting that may a biomarker for However, biological role regulatory mechanism hepatocellular (HCC) remain unknown. In this study, we found was upregulated HCC tissues predicted unfav...