The impact of sperm DNA fragmentation on assisted reproductive technology (ART) successes, in terms of outcome, is now established. High levels of DNA strand breaks severely affect the probability of pregnancy. The importance of sperm nucleus condensation in early embryogenesis and, subsequently, on the quality of the conceptus is now emerging. In this article we have compared in situ analyses ...

Abstract Non-small cell lung cancer (NSCLC) is one of the most lethal malignant tumors. It has been shown that general anesthetic agents, propofol and astragaloside IV (AS-IV) both exert antitumor effects in NSCLC. However, combination with AS-IV NSCLC remain unclear. Cell counting kit-8, EdU Transwell assays were performed to evaluate viability, proliferation, migration. apoptosis autophagy ob...

Epidermolysis bullosa simplex with migratory circinate erythema (EBS-MCE) is a rare EBS subtype characterised by migratory blistering lesions that resolve with brownish pigmentation. It is caused by a recurrent readthrough mutation, c.1649delG, in the tail of keratin 5. Here, we report a child with EBS-MCE and investigated the immunologic mechanisms underlying the migratory lesions in this pati...

To investigate the effect of microwave emitted by mobile phones on the rat central nervous system (CNS), in vitro cultured cortical neuronal cells and in vivo rat’s brain were exposed to the electromagnetic waves emitted by a microwave transmitter that mimics the working frequency of mobile phones. Trypan blue staining and terminal deoxynucleotidy transferase-mediated dUTP nick-end labeling (TU...

introduction: repetitive cortical spreading depression (csd) can lead to cell death in immature brain tissue. caspases are involved in neuronal cell death in several csd-related neurological disorders. yet, whether repetitive csd itself can induce caspase activation in adult or juvenile tissue remains unknown. inducing repetitive csd in somatosensory cortices of juvenile and adult rats in vivo,...

Nitidine chloride (NC), a novel benzo[c]phenanthridine alkaloid, induces the growth inhibition of cancer cells. Previously it was demonstrated that SMMC-7721 human hepatocellular carcinoma (HCC) cells are highly susceptible to the antiproliferative effects of NC. However, the specific mechanisms remained unclear. In the present study the pathways of growth inhibition induced by NC in SMMC-7721 ...


 Purpose: To evaluate the role of miR-20a in sevoflurane (SEV)-induced cognitive impairment rats and to elucidate mechanism action.
 Methods: A SEV-induced rat model was developed. The Morris water maze test fear assay were carried out assess impaired learning memory. cellular SEV-impaired developed level measured animal models. TUNEL staining immunoblot performed determine SEV effec...

Abstract There is a relationship between person’s diet and the development prevention of some cancers. Carotenoids are found as various natural pigments in many fruits vegetables. Studies on carotenoids their potential roles carcinogenesis increasing importance day by day. In this study, we aimed to determine cytotoxic genotoxic effects capsanthin, carotenoid compound, human prostate cancer cel...

PURPOSE In Asian countries, laser iridotomy for the treatment of angle-closure glaucoma is a common cause of bullous keratopathy, which may be associated with a shallow anterior chamber and dark iris pigmentation in Asians. Several cases of corneal decompensation after argon laser iridotomy have been reported. In the present study, we evaluated the harmful effects of argon laser iridotomy on th...

By the early 1990s, scientists knew that programmed cell death, or apoptosis, was important for tissue development and homeostasis as well as for cancer progression. Because apoptotic cells activate nucleases to cleave their chromatin, cell death could be followed in vitro by extracting DNA from a population of tissue culture cells and running it out on an agarose gel to look for a characterist...