background: the ultrastructural analysis of cultured follicles could direct us to understand subcellular changes during in vitro culture. objective: this study was done to verify the ultrastructural characteristics of in vitro cultured mouse isolated preantral follicles in co-culture system in the presence and absence of leukemia inhibitory factor (lif) materials and methods: mechanically isola...

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ultrastructural remodelling of Ca s...

The yeast Saccharomyces cerevisiae is a key model system for studying of a multitude of cellular processes because of its amenability to genetics, molecular biology and biochemical procedures. Ultrastructural examinations of this organism, though, are traditionally difficult because of the presence of a thick cell wall and the high density of cytoplasmic proteins. A series of recent methodologi...

The ultrastructure of inner membrane-matrix mitochondria isolated from bean (Phaseolus vulgaris) shoots was examined in different metabolic states. Gross ultrastructural transformations analogous to the condensed-to-orthodox configurational changes reported in mammalian mitochondria are observed on transistion from nonrespiring to respiring metabolism. With the induction of oxidative phosphoryl...

Functional disorders of the gallbladder and sphincter Oddi in children remain relevant due to their significant percentage structure diseases biliary system. One options for choleretic therapy is use ursodeoxycholic acid. Purpose - study effectiveness therapy, namely acid, with functional by determining activity microRNA-378f generation impact on cells muscular layer wall. Materials methods. A ...

Mancozeb, an ethylene bis-dithiocarbamate, is widely used as a fungicide and exerts reproductive toxicity in vivo and in vitro in mouse oocytes by altering spindle morphology and impairing the ability to fertilize. Mancozeb also induces a premalignant status in mouse granulosa cells (GCs) cultured in vitro, as indicated by decreased p53 expression and tenuous oxidative stress. However, the pres...

OBJECTIVE To determine whether there is evidence of increased DNA fragmentation and ultrastructural changes in muscle tissue of patients with fibromyalgia (FM) compared with healthy controls. METHODS Muscle tissues from 10 community residents with FM and 10 age and sex matched healthy controls were examined "blindly" for the presence of DNA fragmentation by two different methods: terminal deo...

The midgut in the freshwater shrimp Neocaridina davidi (previously named N. heteropoda) (Crustacea, Malacostraca) is composed of a tube-shaped intestine and a large hepatopancreas that is formed by numerous blind-ended tubules. The precise structure and ultrastructure of these regions were presented in our previous papers, while here we focused on the ultrastructural changes that occurred in th...

PURPOSE Few complications have been reported for deep lamellar endothelial keratoplasty (DLEK). Endothelial graft failure has rarely been disclosed as a complication. Although the histopathologic and ultrastructural analysis of a failed DLEK graft has been previously described, we are not aware of any reports of these features in a case of traumatic endothelial graft failure. METHODS We repor...

OBJECTIVE The cause of meconium stained amniotic fluid in term healthy pregnancies is not clearly understood yet. The aim of this study was to investigate the placental ultrastructural changes and placental apoptosis in pregnancies complicated with meconium stained amniotic fluid. MATERIAL AND METHOD The study group was composed of mothers (n: 13) and their term, appropriate for gestational a...