We evaluated a commercially available enzyme immunoassay method for determining the concentration of total diazepam plus its active metabolite, nordiazepam, in small amounts of serum and urine. The coefficients of variation were 5% within assay and 8% between assay. Concentrations so measured for patients' sera correlated well with those obtained both by gas chromatography with electron-capture...

The major radiation of the sun can be roughly divided into three regions: ultraviolet, visible, and infrared light. Detection in these three regions is important to human beings. The metal-insulator-semiconductor photodetector, with a simpler process than the pn-junction photodetector and a lower dark current than the MSM photodetector, has been developed for light detection in these three regi...

A simple, rapid, gradient reversed phase Ultra Performance Liquid Chromatographic method with ultraviolet detection (UPLC-UV) was developed for analysis of Albuterol sulfate and its impurities in Albuterol sulfate inhalation solution pharmaceutical formulation. Chromatography was carried out on a Waters Acquity BEH C18 (1.7μm, 100mm.x 2.1mm) column using 0.02 M Potassium Dihydrogen Orthophospha...

In a previous communication [l] we reported a procedure for the serum determination of midazolam using gas chromatography (GC) with a nitrogenselective detector. GC with nitrogen-selective detection resulted in a selective procedure for the direct determination of midaxolam with a sensitivity of 50 ng/ml. We present, in this paper, a procedure for the direct determination of midazolam and its m...

The method for quantitative determination of flunixin and its metabolite 5-hydroxyflunixin in bovine plasma has been developed and validated. Plasma samples were extracted with acetonitrile and analysed by liquid chromatography with ultraviolet detection. Extraction efficiency was tested and optimum pH conditions were established. Limit of the detection for both compounds was 0.03 μg/mL, limits...

Superheated water chromatography (SWC) with ultraviolet detection was applied to the separation of low molecular weight polyethylene glycols (PEGs). PEG oligomers could be detected sensitively when the detection wavelength was set at 190 nm. The effect of column temperature on the separation of PEG oligomers was investigated. The elution time of all PEG oligomers decreased with increase in the ...