n-Ribulose-l , 5-di-P carboxylase, purified from soybeans, had K, values of 0.13 XnM for COS and 0.19 mM for ribulose1,5-di-P under a Nz atmosphere. O2 inhibited 14C02 incorporation by the enzyme and this inhibition was rapidly reversed by Ns. Inhibition was competitive with respect to CO2 and uncompetitive with respect to ribulose-1,5-di-P. The Ki for O2 was 0.8 mM. This O2 inhibition, togethe...

Inosine monophosphate dehydrogenase (IMPDH), a rate-limiting enzyme in the de novo synthesis of guanine nucleotides, is a therapeutic target for anticancer and antiviral agents. Among the 15 different polyphenols examined, curcumin was found to have an inhibitory effect on the IMPDH activity in both a competitive and uncompetitive manner and to suppress the cellular GTP level in HT-29 colon car...

Phosphoenolpyruvate inhibited Escherichia coli NADP-isocitrate dehydrogenase allosterically (Ki of 0.31 mM) and isocitrate lyase uncompetitively (Ki' of 0.893 mM). Phosphoenolpyruvate enhances the uncompetitive inhibition of isocitrate lyase by increasing isocitrate, which protects isocitrate dehydrogenase from the inhibition, and contributes to the control through the tricarboxylic acid cycle ...

PURPOSE This study was aimed to purify and characterize the Protease inhibitor (PI) from a plant Allium sativum (garlic) with strong medicinal properties and to explore its phytodrug potentials. METHODS Allium sativum Protease Inhibitor (ASPI) was purified using ammonium sulphate fractionation and Fast Protein Liquid Chromatography on anion exchanger Hi-Trap DEAE column. The purified protein ...

Uncompetitive N-methyl-d-aspartate (NMDA) receptor antagonists, such as dizocilpine (MK-801) and memantine, bind to the NMDA receptor channel, and block Ca influx. The increase in intracellular concentration of second messengers, such as Ca and cyclic adenosine monophosphate (cAMP), activate the transcription of certain genes. This process is controlled by cAMP response element binding protein ...

As part of a study of the regulation of aspartate aminotransferase isozymes (EC 2.6.1. l), we have investigated the characteristics of inhibition of the anionic and cationic isozymes by u-erythrose 4-phosphate and glycolaldehyde phosphate, the naturally occurring homologues of D-glyceraldehyde 3-phosphate. Erythrose-4-P has been found to be a time-dependent inhibitor of both the cationic and an...