Introduction. Anemia is a frequent problem in hospitalized geriatric patients, and the anemia of chronic disease (ACD) and iron deficiency anemia (IDA) are the 2 most prevalent causes. The aim of the study was to assess the possible role of serum hepcidin in the differential diagnosis between ACD and IDA. Methods. We investigated serum hepcidin, iron status, anemia, and C-reactive protein in 39...

equine infectious anemia (eia) is a contagious disease that is clinically characterized by recurrent episodes of fever, hemolytic anemia, icterus, depression, edema and chronic weight loss. the disease is associated with a persistent viral infection. the virus of eia is a member of the retroviridae family. the aim of this study was to determine the status of eia in horses in ahvaz. one hundred ...

in a comparative study, 460 serum samples were tested for the serological determination of human immunodeficiency vrius (hiv) using an elisa kit and a rapid test kit. results indicated that the rapid test is reliable for screening unkonwn samples when there is no special equipment for screening with immunoblotting and/or elisa.

Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong ad...

The quality of DNA extract significantly influences the outcome of PCR-based detection methods. Performances of four different DNA extraction methods were evaluated for their ability to recover Salmonella DNA from artificially contaminated specimens. Swine faecal and pork samples were spiked with known concentration of Salmonella Typhimurium, DNA was then extracted by each of the methods consid...

Reportedly, complexes between factor XI and ADAMTS13 are detected with a commercial ADAMTS13/FXI ELISA kit in plasma and are decreased in thrombotic thrombocytopenic purpura (TTP). Using this kit, control and TTP patient plasma contained varying amounts of signal (25-670% of a reference plasma) but no signal was observed for mixtures of recombinant enzymes, suggesting little interaction. ADAMTS...

The present study was conducted to compare two stool antigen detection kits with PCR for the diagnosis of Entamoeba histolytica infections by using fecal specimens submitted to the Department of Microbiology at St. Vincent's Hospital, Sydney, and the Institute of Medical and Veterinary Science, Adelaide, Australia. A total of 279 stool samples containing the E complex (E. histolytica, Entamoeba...

OBJECTIVE The aim was to test the performance of a commercial enzyme-linked immunosorbent assay (ELISA) kit for Candida mannan antigen for detecting Candida in oral rinse solutions. STUDY DESIGN Forty-eight oral rinse solutions (38 from patients and 10 from healthy volunteers) were available. Mannan antigen was measured using a commercial sandwich ELISA kit, Unimedi Candida. The result of the...

The establishment of human gut microbiota commences initially in utero. Meconium-the first fecal material passed after birth-can be used to study fetal gut contents; however, processing meconium samples for microbiome studies presents significant technical challenges. Meconium hosts a low biomass microbiome, is tar-like in texture and contains high concentrations of PCR inhibitors. This study a...

Sequencing of Buchnera amino acid genes Amplification primers (table S2) were designed using the sequenced genome of Buchnera APS (NC_002528.1) and synthesized by Eurofins-MWG Operon. PCR conditions were an initial denaturing step at 94° C for 2 min, followed by 30 cycles of 94° C for 30 s, 54-60o C depending on primer Tm for 30 s, 72° C for 2-4 min depending on amplicon length, and a final ext...