Transgenic chickens expressing human sequence antibodies would be a powerful tool to access human targets and epitopes that have been intractable in mammalian hosts because of tolerance to conserved proteins. To foster the development of the chicken platform, it is beneficial to validate transgene constructs using a rapid, cell culture-based method prior to generating fully transgenic birds. We...

We are interested in the efficient and reliable numerical solution of parametric multi-scale problems, the multi-scale (parametric) character of which is indicated by ε (μ) if expressed in the general notation of (1). It is well known that solving parametric multi-scale problems accurately can be challenging and computationally costly for small scales ε and for a strong dependency of the soluti...

The expression of the VH T15 gene product was analyzed in the sera and in the supernatants of polyclonally activated B cells of X-linked immunodeficient (xid) mice. We found that defective males, contrary to normal females, do not express the VH T15 gene product even on antibodies devoid of anti-PC specificity. RNA analysis of polyclonally activated cells with VH specific probes revealed that x...

IgH rearrangements (VH-D, D-JH) are central to the generation of antibody diversity. The majority of the diversity seen in the third hypervariable region is generated by the D segment and at the joints formed by both junctional and N segment variation during D-JH and VH-D rearrangements. The mechanisms that regulate rearrangement are thought to obey the 12/23 rule, wherein D-D or VH-JH rearrang...

Some of the soft SUSY breaking parameters in hidden sector supergravity model depend on the expectation value of the hidden sector scalar potential, 〈Vh〉, whose tree level value is equal to the tree level cosmological constant. The current practice of calculating soft parameters assumes that 〈Vh〉 = 0. Quantum correction to the cosmological constant can differ from the correction to 〈Vh〉 by an a...

In this section, we allow consumers’ base valuations for the product to be uniformly distributed on the interval [vl, vh], independent of consumer type (socially conscious or non-socially conscious), where V = vh vl is defined as the width of the consumer valuation distribution. Socially conscious consumers are assumed to have homogenous, additive willingness-to-pay for responsibility equal to ...

Although the antigen-binding pocket of all antibodies consists of VL + VH dimers (where VL and VH represent immunoglobulin light and heavy chain regions, respectively), subgroups of their VH largely determine their antigen-binding specificities. This VH subgroup dependence automatically relegates subsidiary roles to VL as a whole and to the complementarity-determining region 3 (CDR-3) of VH enc...

BACKGROUND The relationship between plasma biomarkers and vulnerable plaque is not well understood. METHODS AND RESULTS The 188 patients who underwent 3-vessel virtual histology (VH) intravascular ultrasound (IVUS) with peripheral blood sampling were enrolled. Plasma levels of matrix metalloproteinase 2 and 9 (MMP-2, -9), tissue inhibitor of metalloproteinase-1, adiponectin, and macrophage mi...

Here we show that suppression of VH-DJH rearrangement in mice bearing a mu heavy (H) chain transgene (mu-tg mice) is associated with an extended period of DH-JH rearrangement, the first step of Immunoglobulin H chain gene rearrangement. Whereas DH-JH rearrangement is normally initiated and completed at the pro-B cell stage, in mu-tg mice it continues beyond this stage and occurs most frequently...

Previous studies have suggested that murine T cells are tolerant to epitopes derived from germ line variable regions of immunoglobulin (Ig) heavy (VH) or light chains. This has lead to the prediction that germ line VH-region epitopes found in neoplastic B cells cannot be used to provoke an antitumor immune response. To test these assumptions and address the question of how such a vaccine may al...