The expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) and type VIII collagen was studied in human arteries. GM-CSF and type VIII collagen were codistributed in all layers of the walls of nondiseased arteries and during early atherogenesis with up to type V lesions. The number of cells expressing both mRNAs increased during the development of advanced atherosclerotic lesion...

The synthesis, processing, and secretion of factor VIII expressed from heterologous genes introduced into Chinese hamster ovary cells has been studied. The results show factor VIII to be synthesized as a primary translation product of approximately 230 kDa that can be detected in the lumen of the endoplasmic reticulum. In this compartment, the majority of the factor VIII is in a complex with a ...

Factor VIII (antihemophilic factor) has been purified approximately 500.fold from the cryoprecipitate fraction of human plasma. The isolation procedure involves adsorption of contaminants with Al(OH),, column chromatography on tricalcium citrate-cellulose, precipitation with concanavalin A, and an agarose gel filtration step. The linal product is homogeneous when examined by zone electrophoresi...

Both GO (7,8-dihydro-8-oxoguanine) and hoU (5-hydroxyuracil) are highly mutagenic because DNA polymerase frequently misincorporates adenine opposite these damaged bases. In Escherichia coli, MutY DNA glycosylase can remove misincorporated adenine opposite G or GO on the template strand during DNA replication. MutY remains bound to the product that contains an AP (apurinic/apyrimidinic) site. En...

We report on the detection of Ne viii in an intervening multiphase absorption line system at z = 0.32566 in the FUSE spectrum of the quasar 3C 263 (zem = 0.646). The Ne viii λ770 Å detection has a 3.9 σ significance. At the same velocity we also find absorption lines from C iv, O iii, O iv and N iv. The line parameter measurements yield log [N(Ne viii) cm] = 13.98 −0.13 and b = 49.8± 5.5 km s ....

1. A preparation of the specific polysaccharide of Type VIII pneumococcus is described in which the use of heat, strong acid, and alkali were avoided. 2. Quantitative estimations are given of the homologous and cross reacting precipitin and agglutinin in Types III and VIII antisera produced in the rabbit and in the horse. Quantitative data are also given on the mechanism of the Type VIII precip...

The rising incidence of neutralizing antibodies (inhibitors) against therapeutic factor VIII prompted the conduct of studies to answer the question as to whether this rise is related to the introduction of recombinant factor VIII products. The present article summarizes current opinions and results of non-clinical and clinical studies on the immunogenic potential of recombinant compared to plas...

Two hemophilic beagles were given normal sibling. During observation 1200 A whole-body irradiation followed periods of 7 and 24 mo, there was no by a successful marrow graft from a evidence of factor VIII synthesis. ORTHOTOPIC TRANSPLANTATION of a normal liver into a hemophilic dog results in complete correction of the deficiency of factor VIII.”3 However, there are significant extrahepatic sou...

Therapeutic effectiveness of factor VIII inhibitor bypassing materials has been evaluated in dogs with hemophilia A. A standardized template gingival biopsy was performed using local anesthesia. Hemophilic dogs bled extensively from the biopsy site, whereas in normal dogs the wound was sealed within 5 + /- 2 min. If untreated, the hemophilic dogs frequently bled for several days. Factor VIII in...

normal VIII-related protein, as described by Holmberg and Nilsson (1973). The three patients to whom cryoprecipitate was given all showed a postinfusion correction of Ristocetin aggregation, although the response of factor VIII and VIII-related protein varied in each case. To the standard definition of classical von Willebrand's disease, it is now possible to add abnormal Ristocetin-induced agg...