Diagnostic virus isolation is still frequently used, particularly from respiratory tract secretions. Testing positive virus cultures for all possible viruses is time-consuming, and unexpected or unknown viruses may escape detection. Therefore, a novel random PCR approach was developed that allows sequence-independent amplification of viral nucleic acids from virus isolation-positive cultures. S...

Exposure of free-ranging white-tailed deer (Odocoileus virginianus) and mule deer (Odocoileus hemionus) in western Nebraska to selected livestock pathogens was determined by serology and attempted virus isolation. Antibodies to bluetongue virus, epizootic hemorrhagic disease virus, and bovine respiratory syncytial virus were present in both species of deer. No serologic reactors to Brucella or ...

1. Reisen WK. Epidemiology of St. Louis encephalitis virus. Adv Virus Res. 2003;61:139–83. http://dx.doi. org/10.1016/S0065-3527(03)61004-3 2. Causey OR, Shope RE, Theiler M. Isolation of St. Louis encephalitis virus from arthropods in Pará, Brazil. Am J Trop Med Hyg. 1964;13:449. 3. Mettler NE, Casals J. Isolation of St. Louis encephalitis virus from man in Argentina. Acta Virol. 1971;15:148–5...

To the Editor: Free-range domestic ducks can be a key factor in regional spreading of Asian subtype H5N1 avian infl uenza (AI) virus (1–3). Even asymptomatic domestic ducks can shed the virus continuously from the oral cavity and cloaca (3–5). Therefore, early detection of infected ducks that are shedding the virus would reduce the risk of spreading AI virus (H5N1) in a region where the virus h...

To validate the feasibility of using next-generation sequencing in an African context, the complete genome of a rabies virus isolated from a human patient was obtained by high-throughput sequencing after virus isolation in mice and random unbiased amplification. Phylogenetic analysis suggested that this virus belongs to the Africa II clade.

An outbreak of viral encephalitis occurred in Gorakhpur, India, from July through November 2005. The etiologic agent was confirmed to be Japanese encephalitis virus by analyzing 326 acute-phase clinical specimens for virus-specific antibodies and viral RNA and by virus isolation. Phylogenetic analysis showed that these isolates belonged to genogroup 3.

We report here the complete genome sequence of a novel reassortant H4N2 avian influenza virus strain, A/duck/Guangxi/125D17/2012(H4N2) (GX125D17), isolated from a duck in Guangxi Province, China in 2012. We obtained the complete genome sequence of the GX125D17 virus isolation by PCR, cloning, and sequencing. Sequence analysis revealed that this H4N2 virus strain was a novel reassortant avian in...

In 1985 a patient was described whose persistent polyarthritis was found to be aetiologically linked to rubella virus infection through the detection of repeated maximal synovial lymphocyte proliferative responses to rubella virus antigen and by isolation of rubella virus from her synovium. Follow up over the succeeding seven years has shown continuing chronic polyarthritis and persistent synov...

To determine whether Lassa virus was circulating in southern Mali, we tested samples from small mammals from 3 villages, including Soromba, where in 2009 a British citizen probably contracted a lethal Lassa virus infection. We report the isolation and genetic characterization of Lassa virus from an area previously unknown for Lassa fever.

Virus isolation tests on 72 sudden unexplained infant deaths and 34 cases of explained death showed that 42 and 29% respectively had virus infections. A wide range of viruses was encountered, mainly enteroviruses and adenoviruses, mostly from bowel specimens. The findings did not suggest that overwhelming virus infection was a common feature of sudden death in infancy.