The vitellogenin receptor (VgR) functions as an essential component in uptaking and transporting vitellogenin (Vg) in female adults, which is involved in ovary development and oviposition. This study aimed to clarify the molecular characteristics and function of VgR in the oriental fruit fly Bactrocera dorsalis (Hendel). Here, we identified the full-length of BdVgR (GenBank Accession No. JX4691...

The purpose of this study was to verify the hypothesis of feminisation of the freshwater mussel Elliptio complanata exposed for one complete reproductive cycle (1 year) to a municipal effluent plume. Mussels were harvested from a reference lake and placed in specially-designed cages adapted for long-term exposure to a suspended matter-rich river environment. After exposure, mussels were collect...

Vitellogenin was obtained from three year-old vitellogenic trout. Two procedures of isolation were compared: dialysis against distilled water and ultracentrifugation in the density interval 1.21-1.28 g/ml. Similar patterns were observed by gel filtration and electrophoresis for both preparations of vitellogenin, indicating that electric charge and molecular weight were not modified by either pr...

The capacity of cecropia vitellogenic follicles to form yolk during short-term in vitro incubation in female blood was analyzed by labeling with fluorescein-conjugated serum globulin, tritiated cecropia blood proteins, or tritiated amino acid. As judged by fluorescence microscopy or autoradiography, yolk formation during 3-8 hr in vitro was similar in rate and in protein uptake specificity to t...

We report the fate of different derivatives of the vitellogenin genes after injection into fertilized eggs of Xenopus. We injected a constructed minigene as well as a 5' fragment of the A2 vitellogenin gene. The minigene survives in embryogenesis much better than the 5' A2 fragment and is retained more frequently and at a higher level in frog tissues. The mosaic distribution of the foreign DNA ...