F OUR trials were conducted with boar spermatozoa to determine the effects of dilution rate, seminal plasma content and extender composition on susceptibility to cold shock and the development of resistance to cold during 30 C incubation. Acrosome morphology and sperm motility were used to assess damage by cold shock. The susceptibility of boar spermatozoa to cold shock increased as the dilutio...

AIM The aim of the study was to evaluate the effect of washing on the post-thaw quality of ram cauda epididymal spermatozoa (P1: Unwashed, P2: Washed). MATERIALS AND METHODS Fresh testicles of adult healthy slaughtered rams were collected and transported to the laboratory in an ice chest, where they were weighed, and cauda epididymides were separated. These cauda epididymides were used for re...

Background: Idiopathic causes of infertility is associated with oxidative stress. Antioxidants are known to scavenge the excessive production reactive oxygen species (ROS). Green tea (Camellia sinensis) contains polyphenols that enhance its antioxidant potential.Aim: This study focused on impact aqueous green extract normozoospermic human spermatozoa.Setting: Department Medical Biosciences, Uni...

Background: The objective of the current study was to determine the effect of activation of injected oocytes with dithiothreitol on development of ovine embryos derived from in vitro-matured oocytes and intracytoplasmic sperm.The injected oocytes with non-treated sperm were activated with 1) ionomycin (Io) + 6-dimethylaminopurine (6-DMAP) 2) DTT, 3) DTT+ ionomycin (I)+6-DMAP. Materials and Meth...

PURPOSE Because varicocele is seen often in infertile men and oxidative stress has been implicated in sperm dysfunction, we assessed spermatozoal reactive oxygen species and seminal total antioxidant capacity in men with and without varicocele. MATERIALS AND METHODS Levels of reactive oxygen species and total antioxidant capacity were measured in the semen of 21 infertile men with varicocele,...

Introduction Cissampelos capensis, is commonly known by the Afrikaans name ‟dawidjies” or ‟dawidjieswortel”. C. capensis is the most important and best known medicinal plant of the family Menispermaceae used by the Khoisan and other rural people in the western regions of South Africa. Among numerous other ailments, it is traditionally taken to treat male fertility problems. Yet, no studies have...