Fludarabine-containing combinations have additive cell killing against leukemic blasts in vitro. It has also been shown that imatinib mesylate combined with fludarabine or cladribine had an additive effect on CML CFU-GM cells. In this regard, we aimed to investigate the effect of fludarabine-imatinib mesylate combination against CML blastic phase cell lines K562 and Meg-01. XTT test was perform...

Bactericidal antibiotics are known to induce bacterial cell death through the production of reactive oxygen species, which can be neutralized by bacterial oxidative stress control proteins such as catalases. In Escherichia coli, the katE gene encoding catalase is induced by the stringent response pathway. Stringent response is often activated under nutrient and amino acid starvation such as iso...

The present study aimed to prepare A. goniocephala chloroform extract and evaluate antioxidant cytotoxic effects. Then, the nanoparticles (NPs) were synthesized on most efficient extracts biological activities of free forms compared with NPs forms. Antioxidant capacities 14 by column chromatography found FRAP, DPPH CUPRAC methods. Again, all fractions evaluated MCF-7 HT-29 cell lines using XTT ...

BACKGROUND Inhibition of the proteasome-ubiquitin pathway has shown to exert growth inhibitory effects on several human carcinoma cell lines. In this study, the influence of bortezomib on human neuroblastoma cells was investigated. MATERIALS AND METHODS Cell proliferation of seven human neuroblastoma cell lines under bortezomib treatment was assessed by a colorimetric XTT-based assay. Subsequ...

We studied the effect of noninvasive radiofrequency-induced hyperthermia on the viability of Aspergillus fumigatus hyphae in vitro. Radiofrequency-induced hyperthermia resulted in significant (>70%, P < 0.0001) hyphal damage in a time and thermal dose-dependent fashion as assessed by XTT [(sodium 2,3,-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl] (1)-2H-tetrazolium inner salt)...

The paper deals with some possible applications of Knowledge Engineering methods to the practical Software Engineering. Such an integration could provide a way to overcome some constant problems present in the contemporary Software Engineering. This paper describes foundations of the HEKATE Project, which aims at incorporating well established Knowledge Engineering tools and paradigms into the ...

During the screening of the natural products for their ability to inhibit the binding of REV (regulation of virion expression) protein to [33P] labeled RRE (REV responsive element) RNA, two novel fungal metabolites, harziphilone and fleephilone, were isolated from the butanol-methanol (1:1) extract of the fermentation broth of Trichoderma harzianum by bioassay guided fractionation. The structur...

A novel family of 3-((4-oxo-4H-chromen-3-yl)methyl)-4H-chromen-4-one (bis-chromone) derivatives were designed, synthesized and studied for their anti-cancer activity using the XTT assay for the growth inhibition against various human cancer cells. Among them, 3-((5-(cyclohexylmethoxy)-4-oxo-4H-chromen-3-yl)methyl)-7-methoxy-4H-chromen-4-one and 3-((5-(cyclohexylmethoxy)-4-oxo-4H-chromen-3-yl)me...

BACKGROUND The formation of biofilms is crucial in the pathogenesis of many acute and subacute microbial infections, including chronic wounds and foreign-body-related infections. Topical antimicrobial therapy with chemical antiseptics or physical treatment with tissue-tolerable plasma (TTP) may be promising to control bacterial infection. METHODS We assessed the efficacy of 0.1% chlorhexidine...