Abstract Biochar application and conservation tillage are significant for long-term organic carbon (OC) sequestration in soil enhancing crop yields, however, their effects on native (native SOC) without biochar situ remain largely unknown. Here, an 11-year field experiment was carried out to examine different rates (0, 30, 60, 90 Mg ha −1 ) SOC pools labile pool I II, recalcitrant microbial act...