By utilizing a homologous transient expression system, we have demonstrated that the Opaque-2 (O2) gene product O2 confers positive trans-regulation on a 22-kD zein promoter. This trans-acting function of the O2 protein is mediated by its sequence-specific binding to a cis element (the O2 target site) present in the 22-kD zein promoter. A multimer of a 32-bp promoter fragment containing this O2...

Eighty-five cDNA clones for gamma-zein (proline-rich zein) from a cDNA expression library were isolated using specific antibody and cDNA probes. Nucleotide sequences of seven independent clones were determined and found to be identical in regions where they overlapped. The primary structure of the mature protein, determined from the sequence of one near full-length clone, consists of 204 amino ...

Biohybrid materials based on the intercalation of zein, the major storage protein in corn, into sodium-exchanged montmorillonite were prepared following two synthesis strategies. The first one made use of zein dissolved in 80% (v/v) ethanol/water solution, the usual solvent for this protein, while the second method is new and uses a sequential process that implies the previous separation of zei...

Ipomoea turpethum R. Br. [= Operculina terpethum (Linn.) Silva Manso (Hindi—Nisoth, Pitohri), a perennial plant with a muchtwisted stem is found through out India. It is reported to be highly medicinal and is cultivated occasionally in gardens as an ornamental plant. Some hydroxy fatty acids, butulin, lupeol and sitosterol have been found in the plant. Seed gums are important vegetable products...

Abstract The present work demonstrates the new nanofiber mats prepared through co-electrospinning of two different polymers i.e. corn protein namely Zein and Nylon-6. composite membrane was used as an effective adsorbent material for removal toxic reactive dye Reactive Blue 19 (RB19) from water solution. These co-electrospun nanofibers had good mechanical strength compared to zein alone. Experi...