Physicochemical studies of the human serum albuminCu(II)-L-histidine (HSA-Cu(II)-L-His) ternary complex at pH 7.5 indicate several interesting features. 1. The dissociation constants of the HSA-Cu(II)-L-His ternary complex and the HSA-Cu(I1) binary complex are 1.38 X 1O-22 and 6.61 x lOen, respectively. 2. The absorption spectrum of the ternary complex system has characteristics similar to thos...

Transcranial magnetic stimulation (TCMS) causes leg muscle contractions, but the neural structures in the brain that are activated by TCMS and their relationship to these leg muscle responses are not clearly understood. To elucidate this, we concomitantly recorded leg muscle responses and thoracic spinal cord-evoked potentials (SCEPs) after TCMS for the first time in 10 awake, neurologically in...

and Applied Analysis 3 Affine transformation element to b set a = In t+1 Mapping from a a b X = {I1 t+1, I2 t+1, . . . , In t+1} I = {I1 (t+1)(x0,y0), . . . , I 1 (t+1)(xn,yn), . . . , I n (t+1)(xn,yn)} b = {I1 (t+1)(x,y), . . . , I1 (t+1)(x,y)} Figure 2: Affine transformation from X to I. the transfer of the probability state of the affine transformation parameters is obtained, the function of...

Apomyoglobin folding proceeds through a molten globule intermediate (low-salt form; I1) that has been characterized by equilibrium (pH 4) and kinetic (pH 6) folding experiments. Of the eight alpha-helices in myoglobin, three (A, G, and H) are structured in I1, while the rest appear to be unfolded. Here we report on the structure and stability of a second intermediate, the trichloroacetate form ...

The elastic I-band part of muscle protein titin contains two tandem immunoglobulin (Ig) domain regions of distinct mechanical properties. Until recently, the only known structure was that of the I27 module of the distal region, whose mechanical properties have been reported in detail. Recently, the structure of the first proximal domain, I1, has been resolved at 2.1A. In addition to the charact...

The importance of endogenous interleukin 1 (II.-1) in resistance to Pneumocystis carinii infection was examined in a SCID mouse model. Naturally acquired pulmonary infection of P. carinii in SCID mice was completely cleared by reconstitution of the infected mice with immunocompetent spleen cells. I1:1 activity in the lung homogenate supernatant of these mice increased significantly after recons...

I1 dynein, or dynein f, is a highly conserved inner arm isoform that plays a key role in the regulation of flagellar motility. To understand how the IC138 IC/LC subcomplex modulates I1 activity, we characterized the molecular lesions and motility phenotypes of several bop5 alleles. bop5-3, bop5-4, and bop5-5 are null alleles, whereas bop5-6 is an intron mutation that reduces IC138 expression. I...

One of the challenges in understanding ciliary and flagellar motility is determining the mechanisms that locally regulate dynein-driven microtubule sliding. Our recent studies demonstrated that microtubule sliding, in Chlamydomonas flagella, is regulated by phosphorylation. However, the regulatory proteins remain unknown. Here we identify the 138-kD intermediate chain of inner arm dynein I1 as ...

Complexes of histidine with Co( 11) in aqueous solution have been examined by proton magnetic resonance spectroscopy. Coordinate interaction of histidine with paramagnetic Co( 11) produces large contact interaction shifts in the histidine proton resonance spectra. Four histidine-Co( 11) complexes have been distinguished : a weak 1: 1 histidine-Co(I1) complex a t low pH ( < 4 ) in which only the...

not conclusive, it suggests an alternative model of TEL-AML1 leukemogenesis (see figure, Model B). In this model, the initiating event (TEL-AML1 fusion) is as rare as the disease itself, implying that a high proportion (perhaps 100%) of babies born with a detectable TEL-AML1 fusion are destined to develop TEL-AML1 ALL. Could newborn screening for TEL-AML1 be considered in this scenario? Assumin...