We report the application of rotor-assisted population transfer (RAPT) to measure the quadrupolar coupling constant (C(q)) for spin 5/2 nuclei. Results from numerical simulations are presented on the magnitude of enhancement factor as a function of frequency offsets, i.e. the RAPT profile. Experimental O17 RAPT profile is traced for the amino acid L-leucine. In addition, results from MQ-MAS exp...

Let i1 ≥ i2 ≥ i3 ≥ 1 be integers. An L(i1, i2, i3)-labelling of a graph G = (V, E) is a mapping φ : V → {0, 1, 2, . . .} such that |φ(u)− φ(v)| ≥ it for any u, v ∈ V with d(u, v) = t, t = 1, 2, 3, where d(u, v) is the distance in G between u and v. The integer φ(v) is called the label assigned to v under φ, and the difference between the largest and the smallest labels is called the span of φ. ...

Neuronal expression of apolipoprotein (apo) E4 may contribute to the pathogenesis of Alzheimer's disease (AD). In studying how apoE expression is regulated in neurons, we identified a splicing variant of apoE mRNA with intron-3 retention (apoE-I3). ApoE-I3 mRNA was detected in neuronal cell lines and primary neurons, but not in astrocytic cell lines or primary astrocytes, from humans and mice b...

Vaccinia virus, the prototypic poxvirus, efficiently and faithfully replicates its ∼200-kb DNA genome within the cytoplasm of infected cells. This intracellular localization dictates that vaccinia virus encodes most, if not all, of its own DNA replication machinery. Included in the repertoire of viral replication proteins is the I3 protein, which binds to single-stranded DNA (ssDNA) with great ...

In this study, we present data that support the presence of two distinct calmodulin binding sites within the angiotensin II receptor (AT(1A)), at juxtamembrane regions of the N-terminus of the third intracellular loop (i3, amino acids 214-231) and carboxyl tail of the receptor (ct, 302-317). We used bioluminescence resonance energy transfer assays to document interactions of calmodulin with the...

Abstract. The problem of legal regulation the financial and economic security at all levels Ukraine’s economy is extremely important. In our opinion, most complicated in system problematic issue limits state intervention economy. Theoretical basis. It necessary to avoid potential detrimental influence on processes, achieve a reasonable combination public administration concurrently with autonom...

Several lines of evidence suggest that G-protein-coupled receptors can adopt different active conformations, but their direct demonstration in intact cells is still missing. Using a fluorescence resonance energy transfer (FRET)-based approach we studied conformational changes in alpha(2A)-adrenergic receptors in intact cells. The receptors were C-terminally labeled with cyan fluorescent protein...

The iSchool Inclusion Institute (i3) is a Research Experience for Undergraduates (REU) program in the US designed to address underrepresentation in the information sciences. i3 is a year-long, cohortbased program that prepares undergraduate students for graduate school in information science and is rooted in a research and leadership development curriculum. Using data from six years of i3 cohor...

In this study, the oxazine 170 perchlorate (O17)-ethylcellulose (EC) membrane was successfully exploited for the fabrication of creatine- and creatinine-sensing membranes. The sensing membrane exhibited a double layer of O17-EC membrane and a layer of enzyme(s) entrapped in the EC and polyurethane hydrogel (PU) matrix. The sensing principle of the membranes was based on the hydrolytic catalysis...