BACKGROUND TTN-encoded titin, CSRP3-encoded muscle LIM protein, and TCAP-encoded telethonin are Z-disc proteins essential for the structural organization of the cardiac sarcomere and the cardiomyocyte's stretch sensor. All three genes have been established as cardiomyopathy-associated genes for both dilated cardiomyopathy (DCM) and hypertrophic cardiomyopathy (HCM). Here, we sought to character...

The RNA initiation sites of the adenovirus IVa2 and major late promoters (MLP) are separated by 210 base pairs and transcribed from opposite DNA strands. We had previously shown that they contained overlapping promoter sequences (V. Natarajan, M. J. Madden, and N. P. Salzman, Proc. Natl. Acad. Sci. U.S.A. 81:6290-6294, 1984). The transcription efficiencies of these two promoters were studied in...

Exposure of a human leukemic cell line HL-60 to 1 % dimethylsulfoxide (DMSO) for 4 days induced myeloid differentiation. DMSO-differentiated HL-60 cells displayed high and low-affinity binding sites for leukotriene B4 (LTB4). The pretreatment of myeloid differentiated HL-60 cells with 1-10 nM LTB4 enhanced superoxide production evoked by 100 nM formyl-methionyl-leucylphenylalanine (fMLP) to 127...

This study reports on the development of a diagnostic expert system – incorporating a multilayer perceptron (MLP) – designed to identify any sub-type of dysarthria (loss of neuromuscular control over the articulators) manifested by a patient undergoing a Frenchay Dysarthria Assessment (FDA) evaluation. If sufficient information is provided describing pathological features of the patient’s speec...

Muscle cells respond to mechanical stretch stimuli by triggering downstream signals for myocyte growth and survival. The molecular components of the muscle stretch sensor are unknown, and their role in muscle disease is unclear. Here, we present biophysical/biochemical studies in muscle LIM protein (MLP) deficient cardiac muscle that support a selective role for this Z disc protein in mechanica...

This paper discusses the empirical evaluation of improving generalization performance of neural networks by systematic treatment of training and test failures. As a result of systematic treatment of failures, multilayer perceptron (MLP) discriminants were developed as discrimination techniques. The experiments presented in this paper illustrate the application of discrimination techniques using...

A total of 344 patients underwent bilateral total knee replacement (TKR) using a different prosthesis on each side. Four knee prostheses were used: anterior and posterior cruciate-retaining (ACL-PCL), posterior cruciate-retaining (PCL), medial or lateral pivot (MLP), and posterior cruciate-substituting (PS). All patients had good or excellent results. The range of movement, relief from pain, al...

In this paper, a method for designing and implementing of Multilayer Percepton (MLP) based on BP algorithm has been suggested. The method has described a MLP on Register Transfer Level (RTL) using VHDL description language and implemented on Field Programmable Gate Array (FPGA) for speech recognition. Firstly, a multiplyaccumulate (MAC) unit, and sigmoid nonlinear function are implemented as a ...