The hydrogen acceptor 3-(4,5-dimethylthiazol-2-yl)-2,5 dipheny Itetrazolium bromide (Mil) is commonly utilized to estimate cellular viability in drug screening protocols. The present investigation was prompted, in part, by observations that reduction of MTT to its colored reaction product, MTT formazan, varied between cell lines and with culture age. A correlation was established between the D-...

The tetrazolium dye, 3-(4,5-dimethylthia/ol-2-yl)-2,5-diphi-iiyltclrazolium bromide (MTT), is reduced by live but not dead cells, and this reaction is used as the end point in a rapid drug-screening assay. It can also be used for accurate determinations of drug sensitivity but only if a quantitative relationship is established between cell number and M l'I forma/an production. We have shown tha...

Cancer is a major cause of death worldwide and causes serious problems in human life. It is developed by uncontrolled growth of a cell or a group of cells. There are many difficulties in treatment of cancer and many researchers are involved in investigating for effective drugs to treat the disease. Caryophyllaceae is a large family of about 86 genera and 2200 herbaceous or subshrub species. The...

Cloves (Syzygium aromaticum) as been used traditional medicine for many years and they possess antibacterial, antifungal antiviral properties. Clove is known its anticancer property on various cancer cell lines well established, but effect OSCC less known. Aim of the study was to determine antioxidant Syzygium aromaticum extract (KB lines) compare same with normal mouse fibroblasts (L292 lines)...

In comparison to monolayer cells, MCTS has been claimed as more suitable candidate for studying drug penetration due to the high resemblance to solid tumors. However, the cultivation of MCTS is cumbersome, time consuming, and most technique fail to generate spheroids with uniform sizes. Therefore, the application of spheroid cultures in high throughput screening has been rather limiting. Beside...

Background: Catechins are good free radical scavengers but exhibit low stability and permeability. Nanophytosomes currently being developed as the delivery system for phytoconstituents to protect them from decomposition by oxidants or enzymes increase their Objectives: To formulate gambier catechin-loaded nanophytosomes perform MTT assay on HeLa cell lines. Method:Five formulations were prepare...

We investigated the effect of epigallocatechin-gallate (EGCG), the main constituent of green tea polyphenols, on human glioblastoma cell lines U-373 MG and U-87 MG, rat glioma cell line C6, and rat nonfunctioning pituitary adenoma cell line MtT/E. Cell viability was determined by assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and the extent of apoptosis was stud...