BACKGROUND Members of the Parvovirus genus cause a variety of diseases in mammals, including humans. One of the major defences against viral infection is the presence of neutralizing antibodies that prevent virus particles from infecting target cells. The mechanism of neutralization is not well understood. We therefore studied the structure of canine parvovirus (CPV) complexed with the Fab frag...

The size and antigenic relationships among structural proteins (VPs) of canine parvovirus (CPV), feline parvovirus (FPV), porcine parvovirus (PPV), minute virus of mice (MVM) and bovine parvovirus (BPV) were determined by SDS-PAGE of radiolabelled, purified virus and immunoprecipitated viral proteins. Mature virions of CPV, FPV, PPV and MVM were composed of three VPs designated VP1, VP2 and VP3...

We have mapped and determined the nucleotide sequence of the cowpox virus (CPV) gene coding for the protein of A-type inclusion bodies (ATI). The ATI gene was mapped to the central part of the largest HindIII restriction fragment of the CPV genome. Vaccinia virus (VV) also contained a similar sequence at the equivalent position in its genome, which even under stringent conditions hybridized wit...

Complex trapping blocking (CTB) enzyme-linked immunosorbent assays (ELISAs) and indirect ELISAs for the detection of antibodies to canine parvovirus (CPV), canine coronavirus (CCV) and rotavirus in sera of dogs were established. Double antibody sandwich ELISAs for the detection of CPV-, CCV- and rotavirus antigens in fecal samples were also developed. Both the serological and antigen-detection ...

This thesis addresses the problem of verifying the geographic locations of Internet clients. First, we demonstrate how current state-of-the-art delay-based geolocation techniques are susceptible to evasion through delay manipulations, which involve both increasing and decreasing the Internet delays that are observed between a client and a remote measuring party. We find that delay-based techniq...

canine parvovirus type 2 (cpv-2) is one of the most common viruses responsible for acute hemorrhagic enteritis in dogs. a rapid and accurate diagnosis of cpv-2 infection is especially important in kennels in order to isolate infected dogs. the aim of the present study was to compare two laboratory tests i.e., polymerase change reaction (pcr) and immunochromatography assay (ica) most commonly us...