• Kindlin-3–b2-integrin signaling in neutrophils is involved in regulation of both neutrophil recruitment and NET release. • Disrupting the crosstalk between kindlin-3 and b2-integrins in neutrophils with a blocking peptide preferentially attenuates NET release. Kindlin-3 essentially supports integrin activation in blood cells. Absence of kindlin-3 in humanscauses leukocyte adhesiondeficiency-I...

PURPOSE To determine whether COX-2 expression is associated with clinicopathological parameters, including c-erb-B2 overexpression and angiogenesis, and the disease-free survival of patients with operable breast cancer. MATERIALS AND METHODS Paraffin-embedded tissue samples were selected from 205 patients surgically resected for breast cancer, between 1991 and 1997, and followed-up for at lea...

Escherichia coli strains causing human extra-intestinal infections may be divided into two groups, B1 and B2 according to the electrophoretic patterns of carboxylesterase B. This study compares the restriction fragment length polymorphism (RFLP) of ribosomal DNA (rDNA) for 45 B1 strains and 45 B2 strains to examine the genetic structure of B2 strains and to distinguish them from B1 strains. The...

It has been suggested that there is a link between epinephrine synthesis and the development of b2-adrenoceptor–mediated effects, but it remains to be determined whether this development is triggered by epinephrine. The aim of this study was to characterize b-adrenoceptor–mediated relaxation and facilitation of norepinephrine release in the aorta of phenylethanolamine-Nmethyltransferase–knockou...

Invasive growth is a major determinant of the high lethality of malignant gliomas. Plexin-B2, an axon guidance receptor important for mediating neural progenitor cell migration during development, is upregulated in gliomas, but its function therein remains poorly understood. Combining bioinformatic analyses, immunoblotting and immunohistochemistry of patient samples, we demonstrate that Plexin-...

In analogy to the structure of rhodopsin, the seven hydrophobic segments of G-protein-coupled receptors are supposed to form seven membrane-spanning alpha-helices. To analyze the topology of the bradykinin B2 receptor, we raised site-directed antibodies to peptides corresponding to the loop regions and the amino and carboxyl terminus of this receptor. We found that a segment with predicted intr...

There is strong epidemiological association between periodontal disease and cardiovascular disease but underlying mechanisms remain ill-defined. Because the human periodontal disease pathogen, Porphyromonas gingivalis (Pg), interacts with innate immune receptors Toll-like Receptor (TLR) 2 and CD36/scavenger receptor-B2 (SR-B2), we studied how CD36/SR-B2 and TLR pathways promote Pg-mediated athe...

A plausible determinant of the specificity of receptor signaling is the cellular compartment over which the signal is broadcast. In rat heart, stimulation of b1-adrenergic receptor (b1-AR), coupled to Gs-protein, or b2-AR, coupled to Gsand Gi-proteins, both increase L-type Ca 21 current, causing enhanced contractile strength. But only b1-AR stimulation increases the phosphorylation of phosphola...

The effects of smoking, aspirin ingestion, and sex differences on bleeding times and bleeding time thromboxane B2 and 6-keto-prostaglandin (PG)F1 alpha production were examined. Nonsmoking men produced more thromboxane B2 (3.99 +/- 0.76 ng/ml) than nonsmoking women (2.13 +/- 0.24 ng/ml). Female smokers produced more thromboxane B2 (5.01 +/- 0.97 ng/ml) than nonsmoking women. Twenty-four hours a...