aphids are among important agricultural pests. the pest's evolutionary tendency towards a loss of taxonomically useful characteristics, as well as morphological plasticity due to host type and as a result of environmental factors, make a morphological identification of aphids difficult, whereas exact identification is indispensable for managment of these pests. thus, modern identification ...

BACKGROUND DNA barcoding is a popular tool in taxonomic and phylogenetic studies, but for most animal lineages protocols for obtaining the barcoding sequences--mitochondrial cytochrome C oxidase subunit I (cox1 AKA CO1)--are not standardized. Our aim was to explore an optimal strategy for arachnids, focusing on the species-richest lineage, spiders by (1) improving an automated DNA extraction pr...

Hypomyces aurantius is a mycoparasite that causes cobweb disease, a most serious disease of cultivated mushrooms. Intra-species identification is vital for disease control, however the lack of genomic data makes development of molecular markers challenging. Small size, high copy number, and high mutation rate of fungal mitochondrial genome makes it a good candidate for intra and inter species d...

Bu çalışmada Kastamonu’nun merkez ilçesinde bir yaban domuzunun nekropsi analizi sonucunda akciğerlerinde belirlenen ve morfolojik identifikasyonla Metastrongylus pudentodectus olarak teşhis edilen akciğer kıl kurtlarının mitokondrial DNA cytochrome oxidase subunit 1 (COX1) gen bölgesi moleküler karakterize edilmiş filogenetik ilişkileri araştırılmıştır. barkodlama analizlerine 10 adet M. pudon...

BACKGROUND The present study was aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human clinical samples using two mitochondrial genes cox1 and nad1 in Iran. METHODS Forty seven human hydatid cysts were collected through surgery from two hospitals in Tehran during 2010-2012. To determine the fertility of protoscoleces, the cyst fluids were subjected ...

Prostaglandins are synthesized from arachidonic acid by the rate-limiting enzyme cyclooxygenase (prostaglandin G/H synthase). Active cyclooxygenase is encoded by two distinct and independently regulated genes, termed cyclooxygenase-1 (cox1) and cyclooxygenase-2 (cox2). In this investigation, we examined the expression of cox1 and cox2 mRNA in rat aorta following balloon deendothelialization (BD...

The lysosomal storage disorder Fabry disease is characterized by excessive globotriaosylceramide (Gb3) accumulation in major organs such as the heart and kidney. Defective lysosomal alpha-galactosidase A (Gla) is responsible for excessive Gb3 accumulation, and one cell sensitive to the effects of Gb3 accumulation is vascular endothelium. Endothelial dysfunction is associated with Fabry disease ...