a rapid, sensitive and reproducible hplc method was developed and validatedfor the analysis of amifostine (amf) and/or its metabolite, wr-1065 inhuman plasma. the method involves the alkylation of free sulfydryl group with iodoacetic acid followed by derivatization of the drug and its metabolite witho-phthaldialdehyde (opa) anduvdetection at 340 nm. the derivatized amf and wr-1065 were eluted i...

Intestinal and testicular toxicity in groups of nonirradiated and irradiated mice were investigated after intraperitoneal injection of aminothiol compounds or saline. Four aminothiols were studied. Three were prodrugs: WR-2721, WR-3689, and WR-151327 and one was the active form of WR-2721: WR-1065. Thirty minutes after injection, the mice were sham-irradiated or bilaterally exposed (whole body)...

PURPOSE This study was designed to validate the Actical activity monitor in middle-aged and older adults of varying body composition to develop accelerometer thresholds to distinguish between light and moderate intensity physical activity (PA). METHODS Nonobese 45 to 64 yr (N = 29), obese 45 to 64 yr (N = 21), and ≥65 yr (N = 23; varying body composition) participants completed laboratory-bas...

A rapid, sensitive and reproducible HPLC method was developed and validatedfor the analysis of amifostine (AMF) and/or its metabolite, WR-1065 inhuman plasma. The method involves the alkylation of free sulfydryl group with iodoacetic acid followed by derivatization of the drug and its metabolite witho-phthaldialdehyde (OPA) andUVdetection at 340 nm. The derivatized AMF and WR-1065 were eluted i...

MAPKs constitute a family of serine/threonine protein kinase that has been highly conserved during evolution, with members found in such diverse organisms as mammals, Xenopus and yeast (Pelech and Shanghera, 1992; Blumer and Johnson, 1994; Davis, 1994; Marshall, 1994; Herskowitz, 1995; Schultz et al., 1995). Their activation requires phosphorylation on both threonine and tyrosine residues (Posa...

A rapid, sensitive and reproducible HPLC method was developed and validated for the analysis of amifostine (AMF) and/or its metabolite, WR-1065 in human plasma. The method involves the alkylation of free sulfydryl group with iodoacetic acid followed by derivatization of the drug and its metabolite with o-phthaldialdehyde (OPA) and UVdetection at 340 nm. The derivatized AMF and WR-1065 were elut...

We thank Brian Hearne from McArthur River Mining (MRM) for providing a response to our paper. We were careful in our paper not to draw any direct link between mining activities and Vibrio infections, and to avoid naming the Mine. The potential relevance of water heavy metal concentration to Vibrio infection was not the focus of our paper, illustrated by the fact that it is not mentioned in the ...