The development of gamma-emitting radiopharma ceuticals that could provide more accurate imaging of regional myocardial perfusion than thallium-20l would be useful in the early detection of coronary disease and assessment of therapy. Our approach to the development of such agents utilizes radiolabeled derivatives of drugs, such as the beta-adrenergic antagonists, that exert a selective pharmaco...

OBJECTIVE Using nuclear medicine imaging, we explored suitable acquisition window parameters for assessing the distribution of iodine-125 radioactive seed implantation. SUBJECTS AND METHODS We studied 30 patients with various tumors (21 of which were liver cancers) who had received iodine-125 radioactive seed implantation and had undergone whole-body scintigraphy. Scintigraphy images were acq...

Fluorescence Microscopy (FM) localization of GFP derivatives provides information – sometimes dynamic about the distribution of proteins in cells and tissues while electron microscopy provides information about the ultrastructural components of cells. Correlative light and electron microscopy (CLEM) integrates these two complementary imaging techniques generating a more complete view of the mic...

A sensitive, simple and reproducible protocol for nanoparticle-assisted laser desorption/ionization mass spectrometry imaging technique is described. The use of commercially available TiO2 nanoparticles abolishes heterogeneous crystallization, matrix background interferences and enhances signal detection, especially in the low mass range. Molecular image normalization was based on internal stan...

The present study aimed to characterize comparatively endothelin-1 (ET-1) receptors in rat tissues by radioligand binding assay using [(125)I]ET-1 and to examine receptor binding after oral administration of bosentan. Significant amount of specific [(125)I]ET-1 binding was detected in the lung, heart, kidney, bladder and cerebral cortex of rats. ET-1, bosentan, ambrisentan, and CI-1020 inhibite...

BACKGROUND Suitable diagnostics could identify patients who might benefit from targeted therapies. Molecular imaging is a promising method estimating the expression of specific molecules in vivo, and the goal of this study was to evaluate a radioiodinated anti-epidermal growth factor receptor (EGFR) human Fab as a molecular imaging agent for diagnosis. MATERIALS AND METHODS Three human tumor ...

Direct extraction and ionization techniques using minute amounts of solvent can be employed for the rapid analysis chemical components in a sample without any preparation steps. This type approach is important mass spectrometry imaging samples with multiple that have different spatial distributions (i.e., biological tissues). To improve resolution such imaging, it necessary to reduce volume del...

Paramagnetic metalloporphyrins were examined for their in vivo bio-distribution and their ability to enhance nuclear magnetic resonance imaging of human tumor xenografts in nude mice. The metalloporphyrins tested were: manganese tetrasodium-meso-tetra(4-sulfonatophenyl)-porphine (MnTPPS); manganese meso-tetra-4-pyridylporphine; and gadolinium meso-tetra-4-pyridylporphine. All exhibited high mol...

We have previously used immunohistochemistry to show that the brain of the hagfish, Myxine glutinosa, contains a rich distribution of natriuretic peptide-immunoreactive elements with the densest distribution occurring in the telencephalon and the diencephalon. In this study, the distribution of (125)I-rat ANP and (125)I-porcine CNP binding sites was determined in the brain of M. glutinosa. The ...