embryo culture were used to produce inter specific walnut hybrid plants between juglans nigra l. and j. regia l. developed ovaries were collected from j. rejia female flowers which were pollinated with j. nigra pollen grain by high dusting on a mature tree. in order to isolate embryos, exocarps were aseptically removed. isolated embryos from ovules at different developmental stages were transfe...

Leaves from in vitro proliferating shoots of cvs. Chandler and Hartley of Persian walnut were cultured on MS and NN media supplemented with BA and NAA. Three light conditions including darkness, low light intensity and modified light intensity were used. Most of the calli were friable and non-embryonic in all three conditions. Rate of callogenesis depended on cultivar, culture medium, light con...

Background and objectives: Hawthorn as an ethno-pharmaceutical plant has been consumed for 2,500 years. Flowers, leaves and fruits of hawthorn are rich in antioxidant and polyphenols and are popular in the treatment of congestive heart failure. Methods: In the present study, by using various media and plant hormones the regeneration and callogenesis capacity of...

background: lemon verbena (lippia citriodora h.b.k.) is an aromatic and medicinal plant of family verbenaceae, which cultivated in the north region of iran. objective: this comparative study was carried out on essential oil content and composition of lippia citriodora h.b.k. from micro-propagated and rooted apical stem cutting plantlets. methods: the micro-propagated plantlets were obtained by ...

In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10-15 year old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) suppleme...

this study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of gisela 6 rootstock at khorasan razavi agriculture and natural resource research center during 2011-2012. in this experiment, the proliferation and rooting were performed in six kinds of culture medium (...

nowadays, tobacco is considered as one of the important world industrial plants. in the present study, for a creation of somaclonal variation, calli were produced on ms medium supplemented by 2 mg/l of naphthalene acetic acid (naa) and then regenerated on ms medium supplemented with 1 mg/l benzyl amino purine (bap) and 0.1 mg/l of naa. to determine correlations among traits and to fit multiple ...

background periodontium may be able to respond to injuries by regeneration via the function of stem cells. objectives this study sought to assess the differentiation of human periodontal ligament stem cells (pdlscs) into osteoblasts in standard osteogenic medium and in a medium supplemented with 1,25-dihydroxyvitamin d3 (calcitriol). materials and methods in this experimental study, pdlscs were...

Tissue culture techniques can be beneficial for quick clonal propagation and production of disease-free plants of purple passion fruit as one of the most important medicinal and ornamental plants. These techniques are essential tools for the production of transgenic plants and high-value phytochemicals. The present study was planned to introduce an efficient in vitro propagation for pu...