We showed previously that UBXD8 plays a key role in proteasomal degradation of lipidated ApoB in hepatocarcinoma cell lines. In the present study, we aimed to investigate the functions of UBXD8 in liver in vivo. For this purpose, hepatocyte-specific UBXD8 knockout (UBXD8-LKO) mice were generated. They were fed with a normal or high-fat diet, and the phenotypes were compared with those of litter...

Brown rice is used as a bait-carrier for the avicide DRC-1339 (3-chloro-4-methylaniline) when baiting blackbirds (Icteridae). In March and April 1996 and 1997, we assessed non-target granivorous bird use of rice-baited plots placed in harvested corn and soybean fields in eastern South Dakota for 168 observation hours. In both years combined, we identified 10 and 14 granivorous species in corn a...

We developed a bioenergetics model for European starlings (Sturnus vulgaris) that estimated mortality from baitings with CU Bird Carrier pellets treated with 1% DRC-1339 Concentrate and diluted 5:1 (untreated:treated pellets). A bioenergetic analysis of heat and radiative energy exchanges between the starling body surface and surrounding environment was converted to daily caloric demand based o...

The action of detergents in the isolation of detergent-resistant membrane fractions from rat brain is reported. Triton X-100 treatment of whole rat brain homogenate at 4 degrees C produced detergent-resistant membranes with a density of 1.07g/ml compared with Brij96 where the density of the membrane was only 1.05g/ml. The DRM fractions isolated using Triton X-100 are considerably heavier than t...

Apolipoprotein B (apoB) truncation-specifying mutations cause familial hypobetalipoproteinemia (FHBL). Lipoprotein kinetics studies have shown that production rates of apoB-100 are reduced by 70-80% in heterozygous FHBL humans, instead of the expected 50%. To develop suitable mouse models to study the underlying mechanism, apoB-38.9-only (Apob(38.9/38.9)) mice were crossbred with Apobec-1 knock...

The very promising results of Na-trans-[RuCl4(1H-indazole)2] (NKP-1339) in clinical studies have fuelled renewed interest in the research and development of ruthenium(III) coordination compounds for cancer therapy. By applying an improved synthetic approach to this class of coordination compounds, six new examples of the general formula (cation)-trans-[RuCl4(azole)2], where (cation) = tetrabuty...

Triton X-100 is one of the most widely-applied man-made non-ionic surfactants. This detergent can hardly be degraded by biological treatment. Hence, a more efficient degradation method is indispensable for the total mineralization of this pollutant. Application of heterogeneous photocatalysis based on a TiO₂ suspension is a possible solution. Its efficiency may be improved by the addition of va...

It has been suggested that intracellular Hyal-1 (hyaluronidase-1), which is considered a lysosomal enzyme, originates via endocytosis of the serum enzyme. To test this proposal we have investigated the uptake and intracellular distribution of rhHyal-1 (recombinant human Hyal-1) by mouse liver, making use of centrifugation methods. Experiments were performed on wild-type mice injected with 125I-...

Mycoplasma pneumoniae cells adhering to glass or Parlodion-coated grids were extracted with Triton X-100. The extracted cells showed a cytoskeleton consisting of a rodlike tip structure and a filamentous network in the cytoplasm. The tip structure was up to 300 nm long and approximately 40 nm wide ending at the distal end in a bleb-like structure, and seemed to consist of filaments arranged in ...

A simple, selective and sensitive spectrophotometric method has been developed for the determination of osmium(VIII) using anthranilic acid as a reagent in the presence of Triton X-100. The molar absorptivity and Sandell’s sensitivity of the violet coloured species are 3.03 × 10 L mol cm and 3.5 ng cm respectively. Beer’s law is obeyed between 0.5-5.5 μg mL of Os(VIII) at 500 nm. The stoichiome...