teria and their relatives,” are a major class of bacteria (Gupta, 2000; Holt et al., 1994; Stackebrandt et al., 1988; Zinder, 1998). This group of predominantly Gram-negative bacteria contains more than 200 genera with a great diversity of phenotypic and physiological attributes. Proteobacteria have been classified based on homology of 16S ribosomal RNA or by hybridization of ribosomal RNA or D...

CDC group IVc-2 is a gram-negative, oxidase-positive, nonfermentative bacillus that has been implicated in human infections, including septicemia and peritonitis. Biochemically it most closely resembles Bordetella bronchiseptica and Alcaligenes sp. Results of cellular fatty acid (CFA) and 16S rRNA gene analysis were combined with biochemical data to assist in identification and classification. ...

In this work a method for building maps representing topographic visualization of bacteria taxonomy is presented. The method uses bacteria genotype information regarding the 16S rRNA ”housekeeping gene”, that represents a stable parts of bacteria genome. In order to test the proposed method, we consider the Gammaprotebacteria class and we build a sequences dataset with 147 type strains, downloa...

Symptoms of huanglongbing (HLB) were reported in São Paulo State (SPS), Brazil, in March 2004. In Asia, HLB is caused by 'Candidatus Liberibacter asiaticus' and in Africa by 'Candidatus Liberibacter africanus'. Detection of the liberibacters is based on PCR amplification of their 16S rRNA gene with specific primers. Leaves with blotchy mottle symptoms characteristic of HLB were sampled in sever...

Genomic analysis has revealed heterogeneity among bacterial 16S rRNA gene sequences within a single species; yet the cause(s) remains uncertain. Generalized transducing bacteriophages have recently gained recognition for their abundance as well as their ability to affect lateral gene transfer and to harbor bacterial 16S rRNA gene sequences. Here, we demonstrate the ability of broad-host-range, ...

In order to develop a species-specific PCR for the detection of Mycoplasma genitalium, the sequence of 1,490 bases of the 16S rRNA gene was determined for M. genitalium G37 (type strain) and four Danish isolates of M. genitalium. The sequences of the four Danish strains, mutually different with respect to their MgPa gene, were 100% homologous, although they carried a single common base substitu...

The transcription of the single ribosomal RNA gene cluster is initiated at an assemblage of promoters five of which were previously mapped in the 910 base pair region in front of the 16S rRNA gene. The nucleotide sequence between positions -1680 and -910 was determined and S1 nuclease mapping revealed therein two sites corresponding to the 5' ends of the primary transcripts. These two presumabl...

Streptococcus pluranimalium as a novel human pathogen has been reported by several authors in various contexts. In all reported cases in humans the microorganism was detected by the Vitek2®. The great advances with the introduction of new analytical techniques such as MALDI-TOF or 16S rRNA analysis enabled a more detailed discrimination of pathogens such as these nonhemolytic streptococci. In t...

Previous studies showed that members of the Burkholderiales were important in the succession of aerobic, molybdenum-dependent CO oxidizing-bacteria on volcanic soils. During these studies, four isolates were obtained from Kilauea Volcano (Hawai'i, USA); one strain was isolated from Pico de Orizaba (Mexico) during a separate study. Based on 16S rRNA gene sequence similarities, the Pico de Orizab...