Animal models of ovarian cancer are crucial for understanding the pathogenesis of the disease and for testing new treatment strategies. A model of ovarian carcinogenesis in the rat was modified and improved to yield ovarian preneoplastic and neoplastic lesions that pathogenetically resemble human ovarian cancer. A significantly lower dose (2 to 5 mug per ovary) of 7,12-dimethylbenz(a)anthracene...

In an attempt to obtain a source of material for chemi cal studies, the binding of 7,1 2-dimethylbenz(a)anthracene (DMBA) was studied in E. coli, B. subtilis, and ascites tumor cells. For the bacteria a dose-dependent binding was found, but the level of binding was low. In the case of the ascites tumor cells in the mouse, binding of DMBA to DNA, RNA, and protein showed a dependence on time afte...

BACKGROUND Afobazole, a new 2-mercapto-benzimidazole derivative, exhibited antimutagenic activity in chromosome aberration tests and antioxidant properties. The aim of this study was to demonstrate the potential chemopreventive effect of afobazole on the level of early biological effects by analysing changes in oncogene and tumor suppressor gene expression. MATERIALS AND METHODS Single intrap...

Objectives: Cromolyn, a potent and safe anti-inflammatory drug, is used as a safe medication for the prophylactic treatment of asthma. The present study explores its anti-inflammatory and anti-angiogenic potential of cromolyn by analysing the expression pattern of inflammatory (NF-κB, COX-2, iNOS, IL-6 and IL-10) and angiogenic (VEGF) in 7, 12-dimethylbenz(a)anthracene (DMBA) induced hamster bu...

Pharmacologic and global gene deletion studies demonstrate that cyclooxygenase-2 (PTGS2/COX-2) plays a critical role in DMBA/TPA–induced skin tumor induction. Although many cell types in the tumor microenvironment express COX-2, the cell types in which COX-2 expression is required for tumor promotion are not clearly established.Here, cell type–specificCox-2 gene deletion reveals a vital role fo...

Apoptosis, also known as programmed cell death, is regulated by a number of inhibitory or stimulatory factors. In addition to the pro- and anti-apoptotic Bcl-2 family proteins, there is also a family of inhibitors of apoptosis protein (IAP). Survivin, a member of this IAP family, is selectively upregulated in most tumours. The objective of the present study was, therefore, to investigate the pr...

The formation of DNA adducts from [3H]-7-hydroxymethyl-12-methylbenz(a)anthracene (7-OHM-12-MBA) and [3H]-7,12-dimethylbenz(a)anthracene (DMBA) in the epidermis of Sencar mice was analyzed. Comparison of Sephadex LH-20 chromatographic profiles of DNA samples isolated from mice treated with DMBA or 7-OHM-12-MBA suggested that the DMBA-treated animals contained DNA adduct(s) derived from the furt...

7,12-Dimethylbenz(a)anthracene (DMBA) is a potent carcinogen that induces immunosuppression of both humoral and cell-mediated immunity in mice and other species. Previous studies have shown that CYP1B1 is required for bone marrow toxicity produced by DMBA in mice. Therefore, the purpose of these studies was to determine whether CYP1B1 was required for spleen cell immunotoxicity. Female C57BL/6N...

The effects of dietary supplementation of flavonol quercetin on both 7,12-dimethylbenz(a)anthracene (DMBA)- and N-nitrosomethylurea-induced mammary cancer in female Sprague-Dawley rats were determined. Quercetin diet was started 1 wk before intragastric instillation of DMBA (65 mg/kg of body weight) or i.v. injection of N-nitrosomethylurea (50 mg/kg of body weight) and was continued during the ...

We reported earlier that replacement of casein by soy protein in the diets rats exposed to carcinogen, dimethylbenz[a]anthracene (DMBA), not only delayed initiation breast tumor but also had a protective effect against development aggressive tumor. The aim this study was elucidate molecular mechanism which offers these beneficial effects. Tumor developed gavage administration single dose 80 mg/...