We examined the activities of class I and II alcohol dehydrogenase isoenzymes in the sera of patients with viral hepatitis using the fluorogenic substrates 4-methoxy-1-naphthaldehyde for class I and 6-methoxy-2-naphthaldehyde for class II. It was found that serum activities of class I and II alcohol dehydrogenase isoenzymes over the course of five weeks of hospitalisation were higher than those...

Synthesis of a double-charged cationic amphiphilic 1,4-dihydropyridine derivative with dodecyl ester groups at positions 3 and 5 the 1,4-DHP ring was performed starting from Hantzsch type cyclization acetoacetate, 2-naphthaldehyde ammonium acetate. Bromination this compound followed by nucleophilic substitution bromine (E)-4-(2-(naphthalen-2-yl)vinyl)pyridine gave desired 1,1?-{[3,5-bis(dodecyl...

A method is described for the detection of 2-keto-4-hydroxyglutarate aldolase activity after electrophoresis of the enzyme on polyacrylamide gels. When gels are incubated with substrate (2-keto-4-hydroxyglutarate), activity is seen as a yellow-colored band due to interaction’ of the product (glyoxylate) with ortho-aminobenzaldehyde and glycine. Positive results have been obtained using either c...