References http://rstb.royalsocietypublishing.org/content/367/1595/1589.full.html#ref-list-1 This article cites 160 articles, 30 of which can be accessed free This article is free to access Subject collections (57 articles) systems biology • (23 articles) plant science • (112 articles) molecular biology • (17 articles) genomics • (9 articles) biotechnology • (41 articles) bioinformatics •...

We have directly measured the contribution of dystrophin to the cortical stiffness of living muscle cells and have demonstrated that lack of dystrophin causes a substantial reduction in stiffness. The inferred molecular structure of dystrophin, its preferential localization underlying the cell surface, and the apparent fragility of muscle cells which lack this protein suggest that dystrophin st...

In order to determine which layers of the corneal stroma bear the stress of the intraocular pressure, 6.0-mm nonpenetrating trephine incisions were made centrally in one eye of each of 16 adult albino rabbits. After epithelial healing, the central corneal thickness was measured over 3 hr at 50 mmHg intraocular pressure in both eyes of the anesthetized rabbits. The animals were then killed and t...

Maytansine, an ansa macrolide now in clinical trials as an antineoplastic drug, is a potent inhibitor of microtubule polymerization. Since microtubules are involved in axoplasmic transport, the effect of maytansine on transport was examined. Fast axoplasmic transport of proteins and the axonal ultrastructure was studied in the vagus nerve of cats exposed in vitro to maytansine. Tritiated leucin...

Teleost embryos have not been successfully cryopreserved. To formulate successful cryopreservation protocols, the distribution and cellular permeability to water must be understood. In this paper, the zebrafish (Brachydanio rerio) was used as a model for basic studies of the distribution to permeability to water. These embryos are a complex multi-compartmental system composed of two membrane-li...

The macromolecular structure of the axon membrane in 26-28-day-old Jimpy mice and control optic nerve were examined with quantitative freeze-fracture electron microscopy. Premyelinated and myelinated axons were observed in control optic nerves, with axonal diameters of premyelinated axons being generally smaller than that of myelinated axons (approximately 0.2-0.4 micron vs approximately 0.5-1....

We have studied the roles of 5,10-methylenetetrahydrofolate (5,10-methylene-H4PteGlu) depletion and dihydrofolate (H2PteGlu) accumulation in the inhibition of de novo thymidylate synthesis by methotrexate (MTX) in human MCF-7 breast cancer cells. Using both a high pressure liquid chromatography system and a modification of the 5-fluoro-2'-deoxyuridine-5'-monophosphate radioenzymatic binding ass...

SUMMARY In order to shorten the time-to-market, Application-Specic Integrated Circuits (ASIC's) are designed from a library of pre-dened layout implementations for register-transfer modules such as multipliers, adders, RAM, ROM, etc. Current approaches to selecting the implementations from the library usually deal with their timing-area estimates and do not consider delay of the intermodule wir...

this investigation was performed according to the widespread application of base metal alloys and few articles published about the marginal integrity of restorations fabricated by these metals.three standard dies of a maxillary first premolar were prepared with a flat shoulder finish line in buccal aspect and chamfer in palatal. one of them left with no change. on the buccal aspect of the secon...