نتایج جستجو برای: 5 elisa
تعداد نتایج: 1253905 فیلتر نتایج به سال:
We report the detection of specific IgA antibodies and the determination of IgG avidity in sequential serum samples from a patient exhibiting significant levels of Toxoplasma-specific IgM antibodies for seven years after the onset of the clinical symptoms of toxoplasmosis. IgM antibodies were detected by an indirect immunofluorescence test and by three commercial enzyme-linked immunosorbent ass...
INTRODUCTION Hepatitis C virus (HCV), which was formerly described as the parenterally transmitted form of non-A, non-B hepatitis (NANBH), becomes a chronic disease in 50% of the cases. HCV can also be transmitted through intravenous drug abuse, sexual , and household contact. Hepatitis C virus is a single stranded RNA virus with some structural relations to the flavivirus family. Nucleic acid ...
ELISA system was integrated into a glass microchip. Antigen-antibody and enzyme reactions were performed on a surface of microbeads which were packed in the chip. By monitoring the enzyme reaction product with a thermal lens microscope, good calibration curve was obtained with short assay time. Moreover, to realize higher throughput assay, a semi-automatic ELISA system with a 32-ch microchip wa...
The need for assay methods of sufficient sensitivity to determine the low concentrations of hormones present in body fluids led to the original development of immunoassays and analogous “binding” (or “ligand”) assays in the late 1950s and early 1960s. These methods depend on the use of a binding agent (also commonly referred to by other terms such as “receptor,” “binding reagent,” and “analyte-...
Hepatitis B core Antigen (or HBcAg) is the major component of the core particles of Hepatitis B virus (or HBV). Particles have a size of 27 nm and contain a circular double-stranded DNA molecule, a specific DNA-polymerase and HBcAg. HBcAg is composed of a single polypeptide of about 17 kD that is released upon disaggregation of the core particles; the antigen contains at least one immunological...
This paper describes an electrochemical enzyme-linked immunosorbent assay (ELISA) on paper-based microfluidic devices. This paper-based immunoassay method has several advantages over conventional ELISA, such as short assay time, low cost, and high sensitivity. The limit of detection (LOD) of rabbit IgG used as a model system is ~ 3.9 fM; this value is lower than the LOD of paper-based colorimet...
Recombinant hemagglutinin (H) of the measles virus (MV) expressed in a mammalian high-expression system based on the Semliki Forest virus replicon was used in an enzyme-linked immunosorbent assay (ELISA) for the detection of specific immunoglobulin M (IgM) and IgG in patients with acute-phase measles. One hundred twelve serum specimens from 70 patients with measles were analyzed. Case definitio...
Measurement of humoral tumor-specific immunity can predict what proteins are specific tumor antigens, be used to evaluate patient diagnosis or prognosis, and function as a method by which one can measure the effects of an immune intervention, such as a vaccine. Antibody assays can easily be adapted to high throughput formats; however, specific reagents needed for assay development often are not...
BACKGROUND Following mass vaccination campaigns in the African meningitis belt with group A meningococcal conjugate vaccine, MenAfriVac (PsA-TT), disease due to group A meningococci has nearly disappeared. Antibody persistence in healthy African toddlers was investigated. METHODS African children vaccinated at 12-23 months of age with PsA-TT were followed for evaluation of antibody persistenc...
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